PMID: 9177183Jun 10, 1997Paper

Heparin-binding properties of selenium-containing thioredoxin reductase from HeLa cells and human lung adenocarcinoma cells

Proceedings of the National Academy of Sciences of the United States of America
S Y Liu, T C Stadtman

Abstract

Mammalian selenocysteine-containing thioredoxin reductase (TR) isolated from HeLa cells and from human lung adenocarcinoma cells was separated into two major enzyme species by heparin-agarose affinity chromatography. The low-affinity enzyme forms that were not retained on heparin agarose showed strong crossreactivity in immunoblot assays with anti-rat liver TR polyclonal antibodies, whereas the high-affinity enzyme forms that were retained by the heparin column were not detected. Both low and high heparin-affinity enzyme forms contained FAD, were indistinguishable on SDS/PAGE analysis, and exhibited similar catalytic activities in the NADPH-dependent DTNB [5,5'-dithiobis(2-nitrobenzoate)] assay. The C-terminal amino acid sequences of 75Se-labeled tryptic peptides from lung adenocarcinoma low- and high heparin-affinity enzyme forms were identical to the predicted C-terminal sequence of human placental TR. These two determined peptide sequences were -Ser-Gly-Ala-Ser-Ile-Leu-Gln-Ala-Gly-Cys-Secys-(Gly). Occurrence of the Se-carboxymethyl derivative of radioactive selenocysteine in the position corresponding to TGA in the gene confirmed that UGA is translated as selenocysteine. The presence of cysteine followed by a reactive seleno...Continue Reading

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Citations

May 27, 1999·Journal of Biomedical Science·V N Gladyshev, D L Hatfield
May 25, 2002·Molecular Plant-microbe Interactions : MPMI·Emmanouil FlemetakisPanagiotis Katinakis
Jun 5, 2002·Annual Review of Biochemistry·Thressa Campbell Stadtman
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·S N Gorlatov, T C Stadtman
May 6, 2008·Free Radical Biology & Medicine·Numsen HailJulian E Spallholz
May 5, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Erik G HaymanJ Marc Simard
Jan 22, 2002·Nutrition and Cancer·R Gopalakrishna, U Gundimeda
Nov 4, 2000·The Journal of Biological Chemistry·Q A SunV N Gladyshev
Jun 23, 2000·Annals of the New York Academy of Sciences·T C Stadtman
Aug 27, 1999·Archives of Biochemistry and Biophysics·S N Gorlatov, T C Stadtman
Feb 3, 1998·Biochemical and Biophysical Research Communications·Y MuroM Hagiwara

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