Heparin causes the accumulation of heparan sulfate in cultures of arterial smooth muscle cells

Archives of Biochemistry and Biophysics
S Potter-Perigo, Thomas N Wight

Abstract

Heparin infusion in an experimental animal model of arterial injury causes a significant increase in the proteoglycan (PG) component of the extracellular matrix in the injured arteries (Snow, A. D., Bolender, R. P., Wight, T. N., and Clowes, A. W. (1990) Am. J. Pathol. 137, 313-330). The mechanisms responsible for this heparin-induced increase in arterial PGs are not understood. To address this question, we have examined the effect of heparin on PG synthesis and accumulation by aortic smooth muscle cells in culture. Heparin causes a dose-dependent increase in the accumulation of PGs while the greatest percentage change among the different types of PGs is a doubling of the amount of heparan sulfate (HS) accumulated in the cell layer. There is also a selective enrichment of HS in the trypsin-resistant component of the cell layer indicating a specific modification in intracellular PGs. This change is due to the accumulation of large HS glycosaminoglycans (GAGs) which elute at K(av) approximately 0.3 on Sepharose CL-6B (Mr approximately 50,000) under dissociative conditions and which are absent from the controls. These GAGs are located inside the cell as is indicated by their retention after heparitinase or trypsin treatment of the...Continue Reading

Citations

Jun 27, 1998·Connective Tissue Research·J D San AntonioL A Pukac
May 6, 2003·Archives of Biochemistry and Biophysics·Susan Potter-PerigoThomas N Wight
Oct 26, 2000·Current Opinion in Lipidology·A Chait, T N Wight
Jul 19, 2002·Journal of Biomedical Materials Research·Dulce Papy-GarciaIsabelle Martelly
Dec 24, 2004·Journal of Cell Science·Isabelle BarbosaDulce Papy-Garcia

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