Heparin Decreases in Tumor Necrosis Factor α (TNFα)-induced Endothelial Stress Responses Require Transmembrane Protein 184A and Induction of Dual Specificity Phosphatase 1.

The Journal of Biological Chemistry
Sara Lynn N FarwellLinda J Lowe-Krentz

Abstract

Despite the large number of heparin and heparan sulfate binding proteins, the molecular mechanism(s) by which heparin alters vascular cell physiology is not well understood. Studies with vascular smooth muscle cells (VSMCs) indicate a role for induction of dual specificity phosphatase 1 (DUSP1) that decreases ERK activity and results in decreased cell proliferation, which depends on specific heparin binding. The hypothesis that unfractionated heparin functions to decrease inflammatory signal transduction in endothelial cells (ECs) through heparin-induced expression of DUSP1 was tested. In addition, the expectation that the heparin response includes a decrease in cytokine-induced cytoskeletal changes was examined. Heparin pretreatment of ECs resulted in decreased TNFα-induced JNK and p38 activity and downstream target phosphorylation, as identified through Western blotting and immunofluorescence microscopy. Through knockdown strategies, the importance of heparin-induced DUSP1 expression in these effects was confirmed. Quantitative fluorescence microscopy indicated that heparin treatment of ECs reduced TNFα-induced increases in stress fibers. Monoclonal antibodies that mimic heparin-induced changes in VSMCs were employed to suppo...Continue Reading

References

Jul 1, 1985·Journal of Cellular Physiology·J J CastellotM J Karnovsky
May 30, 1985·Biochimica Et Biophysica Acta·T BârzuJ Caen
Dec 22, 1998·Journal of Vascular Research·C S Dougherty, L J Lowe-Krentz
Nov 27, 1999·The Journal of Biological Chemistry·S Tsukita, S Yonemura
Jun 14, 2000·American Journal of Physiology. Heart and Circulatory Physiology·V H ThouraniJ Vinten-Johansen
Dec 21, 2000·American Journal of Physiology. Heart and Circulatory Physiology·N AzumaB E Sumpio
Aug 31, 2002·The Journal of Biological Chemistry·Usamah S KayyaliPaul M Hassoun
Oct 12, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Talaibek BorbievAlexander D Verin
May 3, 2006·The Journal of Cell Biology·Pirta Hotulainen, Pekka Lappalainen
Jul 15, 2006·Endothelium : Journal of Endothelial Cell Research·Marianne HamelLinda Lowe-Krentz
Jan 9, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Hera ChaudhuryPaul C Evans
Mar 18, 2010·Journal of Cellular Biochemistry·Cheryl Isleib BlaukovitchLinda J Lowe-Krentz
May 14, 2010·American Journal of Physiology. Cell Physiology·O BermudezC Gimond
Jul 14, 2010·Journal of Cellular Physiology·Meron MengistuLinda Lowe-Krentz
Jul 16, 2010·The Biochemical Journal·Ana Cuadrado, Angel R Nebreda
Jan 11, 2012·Frontiers in Physiology·Grzegorz Sowa
Nov 2, 2012·American Journal of Physiology. Cell Physiology·Andrew KooGuillermo García-Cardeña
Feb 23, 2013·Journal of Cardiovascular Pharmacology·Hui-Bin LiuZhi-Ren Zhang
Mar 19, 2013·Matrix Biology : Journal of the International Society for Matrix Biology·Israel VlodavskyNeta Ilan
Mar 26, 2013·The Journal of Biological Chemistry·Keyang Chen, Kevin Jon Williams
Jan 29, 2014·Vascular Pharmacology·Joyce N GonzalesEvgeny A Zemskov
Feb 1, 2014·Integrative Biology : Quantitative Biosciences From Nano to Macro·Eno E EbongJohn M Tarbell
Mar 13, 2014·Annual Review of Biochemistry·Ding Xu, Jeffrey D Esko
Mar 22, 2014·Seminars in Immunology·Guadalupe Sabio, Roger J Davis
Jun 10, 2014·Journal of Cellular Physiology·Albert C GilottiLinda J Lowe-Krentz
Jun 11, 2014·International Journal of Molecular Sciences·Zhen ZengYing Xue
Jun 24, 2014·Atherosclerosis·Narges AminiPaul C Evans
Nov 19, 2014·Proceedings of the National Academy of Sciences of the United States of America·Nicolas BaeyensMartin A Schwartz
Dec 30, 2014·International Journal of Experimental Pathology·John R CouchmanHinke A B Multhaupt

❮ Previous
Next ❯

Citations

Jan 16, 2016·The Journal of Biological Chemistry·Raymond J PughLinda J Lowe-Krentz
Sep 25, 2017·Frontiers in Physiology·Sara Lynn N FarwellLinda J Lowe-Krentz
Jan 1, 2020·American Journal of Physiology. Cell Physiology·Yaqiu LiLinda J Lowe-Krentz
Jul 27, 2018·Research and Practice in Thrombosis and Haemostasis·Barry KevaneFionnuala Ní Áinle
Aug 6, 2020·Biological Procedures Online·Reza Zolfaghari EmamehMajid Khoshmirsafa

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.