Heparin inhibition of smooth muscle cell proliferation: a cellular site of action

Journal of Cellular Physiology
C F ReillyR Rosenberg

Abstract

The potential of a given amount of heparin to inhibit smooth muscle cell (SMC) proliferation can be increased more than 13 fold if quiescent cultures are pretreated with this mucopolysaccharide for 48 h. The large increase in antiproliferative activity was attributable to a 74% inhibition of the first cell cycle traverse of SMC after serum addition. If the mucopolysaccharide was added to SMC coincident with serum, the initial cell cycle traverse was only suppressed by 27%. In both heparin pretreated and nonpretreated SMC cultures, 48 to 72 h elapsed before substantial inhibition was observed. The inhibitory effects of heparin were reversible and inversely proportional to the starting cell density of the cultures. The effects of known heparin binding proteins on the inhibitory capability of heparin were examined. Neither platelet-derived growth factor (PDGF), low density lipoprotein (LDL), nor platelet factor 4 (PF4) were able to reduce the antiproliferative effects. Heparin retained full biological activity in medium containing serum depleted of all heparin binding proteins by heparin-Sepharose chromatography. These results indicate that heparin does not inhibit growth by preventing serum mitogens or nutrients from interacting ...Continue Reading

References

Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H N AntoniadesC D Stiles
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·C H HeldinA Wasteson
Jun 1, 1977·Journal of Cellular Physiology·D GospodarowiczD L Braun
Nov 1, 1977·Thrombosis Research·S P Levine, L S Krentz
Jul 1, 1985·Journal of Cellular Physiology·D L CochranM J Karnovsky
Apr 1, 1985·The Journal of Cell Biology·L M FritzeR D Rosenberg
Jul 1, 1985·Journal of Cellular Physiology·J J CastellotM J Karnovsky
Jul 1, 1985·Journal of Cellular Physiology·J J CastellotM J Karnovsky
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R RossL Harker
Jul 1, 1974·Analytical Biochemistry·S C MarchP Cuatrecasas
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·L T WilliamsH N Antoniades
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C H HeldinA Wasteson
Aug 1, 1981·The Journal of Cell Biology·J J CastellotM J Karnovsky
May 1, 1980·The Journal of Cell Biology·C GajdusekS M Schwartz

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Citations

Oct 1, 1989·Journal of the American College of Cardiology·B SteinJ H Chesebro
May 1, 1991·Journal of the American College of Cardiology·J H ChesebroV Fuster
May 1, 1991·Journal of the American College of Cardiology·B C BerkR W Alexander
May 1, 1991·Journal of the American College of Cardiology·J W CurrierD P Faxon
Jan 1, 1989·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·G M GiladV H Gilad
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T C WrightJ Campisi
Dec 1, 1991·The Australian and New Zealand Journal of Surgery·P E Norman, A K House
Jan 1, 1993·Mediators of Inflammation·L E SilberE J Kovacs
Jul 1, 1997·American Journal of Respiratory Cell and Molecular Biology·S L LeeB L Fanburg
Jan 1, 1990·American Journal of Respiratory Cell and Molecular Biology·W E BenitzM Bernfield
Oct 22, 2013·Journal of Cataract and Refractive Surgery·Sophie MaedelOliver Findl
May 23, 2003·Journal of Vascular and Interventional Radiology : JVIR·Peter H LinStephen R Hanson
Nov 1, 1988·Journal of Vascular Surgery·M DryjskiT D Bjornsson
Sep 8, 2012·Cell Biology International·Gábor BarnaRudolf Mihalik
Sep 29, 1998·Metabolism: Clinical and Experimental·M KohnoJ Yoshikawa
Oct 12, 2004·Journal of Cataract and Refractive Surgery·Hüseyin BayramlarMehmet Borazan
Jun 3, 2015·Journal of Tissue Engineering and Regenerative Medicine·Rebecca A ScottAlyssa Panitch
Jan 1, 1991·Journal of Biomaterials Science. Polymer Edition·T Avramoglou, J Jozefonvicz
Nov 1, 1990·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·J ThybergB A Bottger
Sep 1, 1991·Circulation·J J PopmaE J Topol

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