PMID: 2356119Jun 11, 1990Paper

Heparin inhibits EcoRI endonuclease cleavage of DNA at certain EcoRI sites

Nucleic Acids Research
J Z ChenL A Herzenberg


Studies presented here demonstrate that heparin inhibits EcoRI endonuclease cleavage of DNA whereas related proteoglycans show no effect. The inhibition occurs at particular EcoRI sites that are near or overlap with palindromic sequences in the murine lambda 5 and Lyt-2 genes. Endogenous heparin from peritoneal mast cells co-isolates with DNA and inhibits digestion of peritoneal cell DNA at the inhibitable sites. Digestion of spleen DNA is inhibited at the same sites when commercial heparin is added prior to digestion. In both cases, the inhibition is abolished by pre-treating the DNA with heparinase. Thus, potential artifacts in restriction fragment length analyses could occur with DNA isolated either from cells that are naturally rich in heparin or from cells to which heparin has been added, e.g., as an anticoagulant.


Jan 25, 1975·Journal of Molecular Biology·M Thomas, R W Davis
Jan 1, 1989·Annual Review of Immunology·Y Kitamura
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R V TantravahiJ H Weis
Sep 1, 1987·Analytical Biochemistry·S GustafsonS S Sommer
Jan 1, 1986·Annual Review of Biochemistry·J R HassellV C Hascall
Jul 15, 1983·European Journal of Biochemistry·M NakamuraD W Nebert


Jan 16, 2010·The Journal of Molecular Diagnostics : JMD·Zhian ZhangWayne M Barnes
Sep 1, 1992·Analytical Biochemistry·J E CoadC E Litz
Feb 23, 2007·Analytical Biochemistry·Changbei MaXiaoyuan Lv
Jan 1, 1997·American Journal of Hematology·S R Florell, G M Rodgers

Related Concepts

DNA, Double-Stranded
Mast Cell
Visceral Peritoneum
Selfish DNA
Southern Blot Result
Restriction Endonuclease RsrI

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Torsion Dystonia

Torsion dystonia is a movement disorder characterized by loss of control of voluntary movements appearing as sustained muscle contractions and/or abnormal postures. Here is the latest research.

Generating Insulin-Secreting Cells

Reprogramming cells or using induced pluripotent stem cells to generate insulin-secreting cells has significant therapeutic implications for diabetics. Here is the latest research on generation of insulin-secreting cells.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Epigenome Editing

Epigenome editing is the directed modification of epigenetic marks on chromatin at specified loci. This tool has many applications in research as well as in the clinic. Find the latest research on epigenome editing here.