Heparin-modified dendrimer cross-linked collagen matrices for the delivery of basic fibroblast growth factor (FGF-2)

Journal of Biomaterials Science. Polymer Edition
M A Princz, Heather Sheardown

Abstract

Tissue integration between a tissue-engineered corneal equivalent and the host eye is of critical importance in ensuring long-term implant success. A novel dendrimer cross-linked collagen scaffold has previously shown good corneal epithelial cell compatibility in vitro particularly when the highly functional dendrimer cross-linkers were functionalized to introduce additional biological groups. Herein we investigated heparinization of these materials and their potential to facilitate the delivery of basic fibroblast growth factor (FGF-2) in an active form, ultimately for use as a corneal tissue scaffold. Collagen gels cross-linked with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) chemistry, and varying amounts of polypropyleneimine octaamine generation 2 (G2) dendimer and heparin were synthesized. Swelling studies and differential scanning calorimetry characterization indicated higher gel stability with the introduction of dendrimer cross-linking, which was not compromised by heparin integration. Dendrimer cross-linked gels with or without heparin gave multiple denaturation peaks, as did the heparinized EDC gels. This is thought to be the result of the heterogeneous cross-lin...Continue Reading

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Citations

Mar 17, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Roberta CensiWim E Hennink
Apr 12, 2012·Journal of Biomedical Materials Research. Part a·M A Princz, H Sheardown
Nov 26, 2010·Macromolecular Bioscience·Nick X Wang, Horst A von Recum
Feb 7, 2020·International Journal of Molecular Sciences·Tianyu XiaChao Sun
Dec 6, 2011·Journal of Biomaterials Science. Polymer Edition·M A Princz, H Sheardown
May 7, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jordi Cabanas-DanésPascal Jonkheijm
Feb 12, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Fernando José Costa BaratélaAlvaro Antonio Alencar de Queiroz
Feb 17, 2010·Biomacromolecules·Liesbeth J De CockBruno G De Geest
Sep 14, 2015·ACS Biomaterials Science & Engineering·Tessa LühmannLorenz Meinel

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