Heparin-releasing scaffold for stem cells: a differentiating device for vascular aims

Regenerative Medicine
Cristiano SpadaccioJorge A Genovese

Abstract

Current limitations of tissue-engineered vascular grafts include timing for the scaffold preparation, cell type, cell differentiation and growth inside the construct, and thrombogenicity of the final device. To surmount these shortcomings, we developed a heparin-releasing poly-L-lactide (PLLA) scaffold using the electrospinning technique, to guide the differentiation of human mesenchymal stem cells towards the endothelial phenotype and to deliver a useful drug in the management of the postimplantation period. The heparin-releasing PLLA scaffold was produced by means of the electrospinning technique in a tubular shape. The scaffold was seeded with human mesenchymal stem cells and cultured for up to 1 week. Cell viability and cytotoxicity assays were performed, and cell differentiation was evaluated by immunofluorescence with confocal microscopy, cytofluorometry and western blotting. Heparin release was assayed by Azure A method and biological effectiveness of the drug was assessed by activated clotting time measurements. The scaffold exhibited a morphology favorable to cell attachment. Heparin release showed an initial burst within the first 24 h, followed by a further sustained release profile. After 48 h of culturing, the cons...Continue Reading

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Citations

Mar 25, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Julien RamierDaniel Grande
Dec 14, 2012·The Journal of Biological Chemistry·Kate A MeadeCatherine L R Merry
Mar 4, 2014·Journal of Materials Science. Materials in Medicine·Julien RamierEstelle Renard
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Apr 23, 2020·Minerva cardioangiologica·Cristiano SpadaccioMario F Gaudino
Jan 15, 2021·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Himanshu Patel
Sep 28, 2017·Journal of Agricultural and Food Chemistry·Peng WenHong Wu

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Methods Mentioned

BETA
scanning electron microscopy
bone marrow aspiration
confocal microscopy
flow cytometry

Software Mentioned

ImageTool
CellQuest
Image
Pro

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