Heparinization of cell surfaces with short peptide-conjugated PEG-lipid regulates thromboinflammation in transplantation of human MSCs and hepatocytes

Acta Biomaterialia
Sana AsifYuji Teramura

Abstract

Infusion of therapeutic cells into humans is associated with immune responses, including thromboinflammation, which result in a large loss of transplanted cells. To address these problems, heparinization of the cell surfaces was achieved by a cell-surface modification technique using polyethylene glycol-conjugated phospholipid (PEG-lipid) derivatives. A short heparin-binding peptide was conjugated to the PEG-lipid for immobilization of heparin conjugates on the surface of human mesenchymal stem cells (hMSCs) and human hepatocytes. Here three kinds of heparin-binding peptides were used for immobilizing heparin conjugates and examined for the antithrombogenic effects on the cell surface. The heparinized cells were incubated in human whole blood to evaluate their hemocompatibility by measuring blood parameters such as platelet count, coagulation markers, complement markers, and Factor Xa activity. We found that one of the heparin-binding peptides did not show cytotoxicity after the immobilization with heparin conjugates. The degree of binding of the heparin conjugates on the cell surface (analyzed by flow cytometer) depended on the ratio of the active peptide to control peptide. For both human MSCs and hepatocytes in whole-blood e...Continue Reading

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Citations

May 24, 2016·Seminars in Immunology·Kristina N EkdahlYuji Teramura
Aug 16, 2016·Carbohydrate Polymers·Heloise Ribeiro de BarrosIzabel C Riegel-Vidotti
Apr 26, 2019·Cell Transplantation·Aleksandra Musiał-WysockaMarcin Majka
May 8, 2018·Current Transplantation Reports·James E SquiresAlejandro Soto-Gutierrez
Sep 20, 2020·Biomolecules·Loredana LeggioNunzio Iraci
Aug 4, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Yuya SatoYuji Teramura
Sep 16, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Yuji TeramuraKazuhiko Ishihara
Sep 17, 2021·Cell Transplantation·Hiroyuki OgasawaraMasafumi Goto

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