Heparinization of gas plasma-modified polystyrene surfaces and the interactions of these surfaces with proteins studied with surface plasmon resonance

Biomaterials
C J van DeldenJ Feijen

Abstract

Polystyrene surfaces obtained by spin-coating a solution of polystyrene in toluene on a gold layer were functionalized with carboxylic acid groups by preadsorption of the sodium salt of undecylenic acid, followed by an argon plasma treatment. A conjugate of albumin and heparin (alb-hep) was covalently immobilized onto the functionalized surface via preactivation of carboxylic acid groups with a water-soluble carbodiimide. The immobilization of alb-hep conjugate and the subsequent interactions of the heparinized surface with antithrombin III (ATIII, a heparin cofactor) and thrombin were monitored with surface plasmon resonance (SPR). The surface concentration of conjugate as determined with SPR deviated quantitatively from the results obtained with radiolabelled conjugate. The difference in surface concentrations of conjugate obtained with the two methods probably originates from the uncertainty of the refractive index of the alb-hep conjugate in the SPR technique. ATIII could be bound to the surface modified with alb-hep conjugate but not to a polystyrene surface modified with albumin. Rabbit anti-human ATIII did bind to the alb-hep surface previously exposed to ATIII, confirming the presence of surface bound ATIII. The alb-hep...Continue Reading

References

Jun 2, 1989·Journal of Immunological Methods·C S Mayo, R B Hallock
Jan 1, 1993·Journal of Biomaterials Science. Polymer Edition·J G TerlingenJ Feijen
Nov 1, 1995·Journal of Biomedical Materials Research·C J van DeldenJ Feijen
Jan 1, 1996·Journal of Biomaterials Science. Polymer Edition·C J van DeldenJ Feijen

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Citations

Sep 8, 2011·Physical Chemistry Chemical Physics : PCCP·Jungmok YouEunkyoung Kim
Feb 21, 2008·Journal of Nanobiotechnology·Caterina MinelliAkiko Yamamoto
May 16, 2002·Analytical Biochemistry·Fuming ZhangRobert J Linhardt
Oct 20, 2012·Clinica Chimica Acta; International Journal of Clinical Chemistry·Yu-Chang TyanShiang-Bin Jong
Aug 4, 2009·ChemSusChem·Marijke Van der Steen, Christian V Stevens
Nov 21, 2007·Journal of Biomedical Materials Research. Part a·Tzu-Wen ChuangFeng-Huei Lin
Jun 1, 2010·Journal of Biomaterials Applications·Anand P KhandwekarMukesh Doble

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