Heparinized Polyurethane Surface Via a One-Step Photografting Method

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Zhangshuan LiuGang Wu

Abstract

Traditional methods using coupling chemistry for surface grafting of heparin onto polyurethane (PU) are disadvantageous due to their generally low efficiency. In order to overcome this problem, a quick one-step photografting method is proposed here. Three heparin derivatives incorporating 0.21, 0.58, and 0.88 wt% pendant aryl azide groups were immobilized onto PU surfaces, leading to similar grafting densities of 1.07, 1.17, and 1.13 μg/cm², respectively, yet with increasing densities of anchoring points. The most negatively charged surface and the maximum binding ability towards antithrombin III were found for the heparinized PU with the lowest amount of aryl azide/anchor sites. Furthermore, decreasing the density of anchoring points was found to inhibit platelet adhesion to a larger extent and to prolong plasma recalcification time, prothrombin time, thrombin time, and activated partial thromboplastin time to a larger extent. This was also found to enhance the bioactivity of immobilized heparin from 22.9% for raw heparin to 36.9%. This could be explained by the enhanced molecular mobility of immobilized heparin when it is more loosely anchored to the PU surface, as well as a higher surface charge.

References

Nov 1, 1988·Journal of Biomedical Materials Research·K D ParkS W Kim
Feb 1, 1994·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Y I WuM A Blajchman
Mar 27, 2001·Journal of Biomaterials Science. Polymer Edition·P OlssonJ Riesenfeld
Mar 12, 2003·Hematology/oncology Clinics of North America·Jeanine M WalengaRodger L Bick
Mar 21, 2003·The New England Journal of Medicine·David C McGee, Michael K Gould
Jun 13, 2003·Journal of Medicinal Chemistry·Robert J Linhardt
Feb 22, 2008·Current Topics in Medicinal Chemistry·Saravanababu MurugesanRobert J Linhardt
Aug 10, 2010·Accounts of Chemical Research·Li-Hong Liu, Mingdi Yan
Oct 7, 2011·Macromolecular Bioscience·Fen RanChangsheng Zhao
Apr 14, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Paola ChiodelliMarco Rusnati
Sep 4, 2015·Angewandte Chemie·Guillaume DelaittreChristopher Barner-Kowollik
Mar 12, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Pierre A J MourierChristian Viskov
Apr 12, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Valentine MinetFrançois Mullier
May 5, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chris C Rider, Barbara Mulloy
May 10, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marcelo LimaEdwin Yates

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Citations

Jan 21, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Liang ShanZhi Ping Xu

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Methods Mentioned

BETA
quartz crystal microbalance
protein assay

Software Mentioned

SPSS

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