Hepatic acyl-CoA:cholesterol acyltransferase. Development of a standard assay and determination in patients with cholesterol gallstones

Clinica Chimica Acta; International Journal of Clinical Chemistry
J L SmithI R Hardie

Abstract

A standard assay was developed for human liver acyl-CoA:cholesterol acyltransferase (ACAT, EC 2.3.2.26) which is more sensitive than previous methods and allows accurate activity determinations on crude microsomal fractions. ACAT activity was measured in microsomes from livers of four gallstone patients and five controls. Preincubation with exogenous cholesterol produced an increase in ACAT activity in all liver samples: gallstone samples showed a mean increase of 1.8-fold, whereas non-gallstone samples showed a mean increase of 8.2-fold. The mean ACAT activity measured in the presence of exogenous cholesterol was 52.8 +/- 22.8 (n = 4) pmol . min-1 . mg-1 for gallstone samples and 82.8 +/- 13.5 (n = 4) pmol . min-1 . mg-1 for non-gallstone samples. These results suggest that patients suffering from cholesterol gallstones have a reduced ability to esterify potentially harmful free cholesterol compared with controls. They support the proposition that cholesterol gallstone formation is related to altered hepatic cholesterol metabolism.

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Citations

Oct 16, 1989·Clinica Chimica Acta; International Journal of Clinical Chemistry·J L SmithI R Hardie
Jun 16, 1993·Clinica Chimica Acta; International Journal of Clinical Chemistry·J L SmithJ de Jersey
Dec 9, 1996·Clinica Chimica Acta; International Journal of Clinical Chemistry·J L SmithJ de Jersey
Mar 10, 2009·Liver International : Official Journal of the International Association for the Study of the Liver·Silvana Zanlungo, Attilio Rigotti
Jan 20, 2004·Journal of Lipid Research·Jeffery L SmithJohn de Jersey
Aug 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S SahlinK Einarsson
Aug 1, 1991·Biulleten' eksperimental'noĭ biologii i meditsiny·Iu A Bogdarin
Oct 26, 2001·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·S ElyR Kisilevsky
Sep 22, 2007·The Journal of Nutrition·Moises Torres-GonzalezMaria Luz Fernandez
Jan 19, 1996·Biochimica Et Biophysica Acta·T TreloarJ de Jersey
May 1, 1991·Trends in Pharmacological Sciences·D R Sliskovic, A D White
Feb 1, 1992·Metabolism: Clinical and Experimental·J DallongevilleS Lussier-Cacan

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