Abstract
Islet xeno-transplantation via the portal vein has been proposed as an alternative of islet allo-transplantation for treatment of type 1 diabetes. However, the precise hepatic capacity has not been addressed. We mimicked cell transplantation by infusion of dogs with alginate/poly-1-lysine/alginate (APA) microbeads via either the portal vein (PV) or hepatic artery (HA). The maximal adaptable capacity for infused microbeads was evaluated by examination of vasculature, microvasculature, hepatic hemodynamics, portal vein, and hepatic artery pressures and liver function in the microbead recipient dogs. PV but not HA dogs demonstrated elevated portal pressure during the infusion procedure in a dose-dependent manner. Four out of twelve PV dogs infused with 32,000 microbeads/kg developed acute liver infarction within 24 h after infusion with four of the remaining eight animals developing portal venous thrombosis within 24 h following infusion. All PV animals demonstrated abnormal alanine aminotransferase (ALT) values, and the extent and duration of increased ALT levels correlated with the increase in the number of microbeads infused. In contrast, HA animals infused with as many as 32,000 microbeads/kg had neither portal thrombosis nor ...Continue Reading
References
Jan 1, 1989·Diabetes·A AnderssonL Jansson
Nov 21, 1980·Science·F Lim, A M Sun
Jul 1, 1981·Radiology·V P Chuang, S Wallace
Apr 15, 1995·Transplantation·A M ShapiroN M Kneteman
Dec 31, 1997·Human Pathology·M K FrobergD E Sutherland
Jul 27, 2000·The New England Journal of Medicine·A M ShapiroR V Rajotte
Nov 30, 2000·Upsala Journal of Medical Sciences·W BennetO Korsgren
Apr 6, 2001·Diabetes·E A RyanA M Shapiro
Jun 28, 2002·Diabetes·Boaz HirshbergDavid M Harlan
Oct 24, 2002·Transplantation·John J CaseyA M Shapiro
Apr 5, 2003·Cardiovascular and Interventional Radiology·Patricia L Moreira, Yuehuei H An
May 26, 2004·Journal of Hepatology·Ira J Fox, Jayanta Roy-Chowdhury
Feb 24, 2006·Nature Medicine·Bernhard J HeringHenk-Jan Schuurman
Feb 28, 2006·Nature Medicine·Kenneth CardonaChristian P Larsen
Aug 2, 2006·Biomaterials·Paul de VosRicardo Calafiore
Aug 12, 2006·Current Molecular Medicine·Federico BertuzziAntonio Secchi
Sep 29, 2006·The New England Journal of Medicine·A M James ShapiroJonathan R T Lakey
Mar 3, 2009·Transplantation Proceedings·A G AbalovichJ Milei
Sep 1, 2006·Seminars in Interventional Radiology·Brian Funaki
Citations
Mar 16, 2012·Journal of Vascular and Interventional Radiology : JVIR·Ron C GabaJose Oberholzer
Dec 17, 2010·Xenotransplantation·Mårten K J Schneider, Jörg D Seebach
Jul 28, 2020·Frontiers in Immunology·Richard W LaingSimon C Afford