Hepatic cells derived from induced pluripotent stem cells of pigtail macaques support hepatitis C virus infection

Gastroenterology
Marion SourisseauMatthew J Evans

Abstract

The narrow species tropism of hepatitis C virus (HCV) limits animal studies. We found that pigtail macaque (Macaca nemestrina) hepatic cells derived from induced pluripotent stem cells support the entire HCV life cycle, although infection efficiency was limited by defects in the HCV cell entry process. This block was overcome by either increasing occludin expression, complementing the cells with human CD81, or infecting them with a strain of HCV with less restricted requirements for CD81. Using this system, we can modify viral and host cell genetics to make pigtail macaques a suitable, clinically relevant model for the study of HCV infection.

References

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Citations

Sep 27, 2014·Viruses·Sara W BirdEric O Freed
Aug 31, 2016·Nature Microbiology·David Paul, Ralf Bartenschlager
Mar 31, 2015·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Margaret A ScullAlexander Ploss
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Related Concepts

CD81 protein, human
Hepatitis C
Macaca nemestrina
Virus Replication
Hepatitis C virus
Hepatocyte
Virus Membrane Fusion
Host-Pathogen Interactions
Human Induced Pluripotent Stem Cells
CD81 antigen

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