Hepatic cells derived from induced pluripotent stem cells of pigtail macaques support hepatitis C virus infection

Marion SourisseauMatthew J Evans


The narrow species tropism of hepatitis C virus (HCV) limits animal studies. We found that pigtail macaque (Macaca nemestrina) hepatic cells derived from induced pluripotent stem cells support the entire HCV life cycle, although infection efficiency was limited by defects in the HCV cell entry process. This block was overcome by either increasing occludin expression, complementing the cells with human CD81, or infecting them with a strain of HCV with less restricted requirements for CD81. Using this system, we can modify viral and host cell genetics to make pigtail macaques a suitable, clinically relevant model for the study of HCV infection.


Jun 11, 2005·Science·Brett D LindenbachCharles M Rice
Aug 19, 2005·Nature·Robert S Brown
Nov 10, 2010·Stem Cells and Development·Bonan ZhongHans-Peter Kiem
Sep 27, 2012·Methods : a Companion to Methods in Enzymology·Gabrielle Vieyres, Thomas Pietschmann


Sep 27, 2014·Viruses·Sara W BirdEric O Freed
Aug 31, 2016·Nature Microbiology·David Paul, Ralf Bartenschlager
Mar 31, 2015·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Margaret A ScullAlexander Ploss
Dec 3, 2014·Journal of Hepatology·Koen VercauterenPhilip Meuleman
May 17, 2014·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Benjamin IsraelowMatthew J Evans
Aug 26, 2015·Current Opinion in Virology·Koen VercauterenPhilip Meuleman
Sep 26, 2013·Genomics, Proteomics & Bioinformatics·Xiao Lu, Tongbiao Zhao
Aug 18, 2016·Hepatology International·Namita Roy-ChowdhuryJayanta Roy-Chowdhury
Aug 22, 2016·Journal of Virological Methods·Atichat KuadkitkanDuncan R Smith
Sep 27, 2016·Nature Medicine·Kristina M Adams WaldorfLakshmi Rajagopal
Nov 19, 2014·Nature Communications·Benjamin IsraelowMatthew J Evans
Sep 25, 2015·Journal of Virology·Caroline MarnataAnnette Martin
Nov 12, 2016·MBio·Markus von SchaewenAlexander Ploss
Apr 15, 2016·Nature Reviews. Gastroenterology & Hepatology·Emmanuel Thomas, T Jake Liang
Aug 19, 2014·Immunogenetics·Julie A KarlDavid H O'Connor
Dec 18, 2019·Cold Spring Harbor Perspectives in Medicine·Alexander Ploss, Amit Kapoor
Jun 6, 2018·Frontiers in Immunology·Rani BurmPhilip Meuleman
Sep 1, 2016·Current Pathobiology Reports·Robert E SchwartzAngela Frankel
Apr 14, 2017·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Xuan GuoJun-Wen Li
Mar 4, 2017·Journal of Immunology Research·Janina BrueningGisa Gerold
Jun 4, 2020·International Journal of Molecular Sciences·Keith A BerggrenAlexander Ploss

Related Concepts

CD81 protein, human
Hepatitis C
Macaca nemestrina
Virus Replication
Hepatitis C virus
Virus Membrane Fusion
Host-Pathogen Interactions
Human Induced Pluripotent Stem Cells
CD81 antigen

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