Hepatic ethoxy-, methoxy- and pentoxyresorufin O-dealkylase activities in Landrace and Duroc pigs stimulated with HCG

Reproduction in Domestic Animals = Zuchthygiene
G ZamaratskaiaØ Andresen

Abstract

The effect of human chorionic gonadotropin (hCG) stimulation on the activities of ethoxyresorufin O-deethylase (EROD), methoxyresorufin O-demethylase (MROD) and pentoxyresorufin O-depentylase (PROD) was studied in intact male pigs of purebred Landrace and Duroc breeds. Pigs were divided into four groups: two control groups of each breed, without hCG stimulation (n = 20 for each breed), and two experimental groups (n = 18 for each breed), with hCG stimulation (Pregnyl(®); N.V. Organon, Oss, The Netherlands, 30 IU/kg live weight). Pigs were slaughtered 3 days after hCG stimulation and enzyme activities were measured in hepatic microsomes using two approaches. First, only one substrate concentration was used for the analysis of each enzyme activity. We found that EROD activity was suppressed by hCG-stimulation in Landrace (p = 0.004), but not Duroc pigs (p > 0.05). Generally, EROD activity was higher in Duroc pigs compared with Landrace (p = 0.017). Methoxyresorufin O-demethylase and PROD activities did not differ between groups (p > 0.05). To further characterize EROD, MROD and PROD, enzyme kinetic studies were performed. V(max) values for EROD and MROD in both breeds were lower after hCG stimulation (p < 0.001 for Landrace and p...Continue Reading

References

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Mar 8, 2008·Reproduction in Domestic Animals = Zuchthygiene·G ZamaratskaiaO Andresen
Aug 1, 2009·Animal : an International Journal of Animal Bioscience·G ZamaratskaiaA Madej

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Citations

Jan 27, 2012·Reproduction in Domestic Animals = Zuchthygiene·G ZamaratskaiaØ Andresen
Nov 20, 2014·Computational and Structural Biotechnology Journal·Martin Krøyer Rasmussen, Galia Zamaratskaia

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