PMID: 7543633Aug 1, 1995Paper

Hepatic function is preserved following liver-directed, adenovirus-mediated gene transfer

The Journal of Surgical Research
K E DrazanA Shaked

Abstract

Inherited and acquired disorders of the liver are attractive targets for gene therapy. Hepatic cells are susceptible targets for shuttle vectors because of a diversity of protein and viral receptors and accessibility of a selective afferent blood supply. Preservation of existing hepatic cell integrity and metabolic function is of paramount importance for successful whole animal gene therapy trials. In this report, we examine hepatic cell function and integrity following adenovirus-mediated reporter gene transfer to the liver in vivo. E1-deleted, replication-defective adenovectors encoding the LacZ gene driven by the human CMV promoter were delivered to the liver by isolated portal perfusion. The gene transfer rate, as determined by specific histochemical staining, approached 30% with recombinant protein detectable by Western blot throughout the course of study. Hepatic cell integrity as assessed by histology and hepatic enzyme profile (serum aspartate aminotransferase, gamma-glutamyl transpeptidase) demonstrated normal cellular architecture and no significant difference between transfected liver and controls. Hepatic synthetic and metabolic function, as determined by albumin levels, prothrombin time, and bilirubin, were similar...Continue Reading

Citations

May 18, 2000·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·Y FutagawaY Eto
Dec 21, 2004·Journal of Korean Medical Science·Jeong-Heum BaekJin-Young Kwak
May 15, 2003·British Journal of Pharmacology·Kazunori ToyodaDonald D Heistad
Feb 2, 1999·Journal of Hepatology·C HellerbrandD A Brenner
Feb 14, 2002·Kidney International·Xuehai YePatricio E Ray
Oct 7, 1997·Molecular and Cellular Biology·Y H LeeF J Gonzalez

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.