Hepatic glucose-6-phosphatase flux and glucose phosphorylation, cycling, irreversible disposal, and net balance in vivo in rats. Measurement using the secreted glucuronate technique

Metabolism: Clinical and Experimental
M K HellersteinS Turner

Abstract

Measurement of hepatic glucose production (HGP) by standard isotope dilution reveals only the net release of glucose from the liver, not the flux across glucose-6-phosphatase ([G6Pase] or total hepatic glucose output), hepatic glucose cycling (HGC), irreversible glucose disposal into glycogen in the liver (hepatic Rd), or net hepatic glucose balance. We describe two independent isotopic techniques for measuring these parameters in vivo, both of which use secreted glucuronate (GlcUA). HGC can be quantified by measuring a correction factor for glucose label retained in hepatic glucose-6-phosphate (G6P), sampled as GlcUA. A complementary technique for measuring total hepatic glucose output is also described (reverse dilution), requiring administration of no labeled glucose but instead a labeled gluconeogenic precursor and unlabeled glucose. Hepatic Rd is calculated by multiplying the rate of appearance (Ra) of hepatic UDP-glucose ([UDP-glc] based on dilution of labeled galactose in GlcUA) times the direct entry of glucose into hepatic UDP-glc and the fraction of labeled UDP-glc retained in the liver. The sum of hepatic Rd plus HGC represents the total hepatic glucose phosphorylation rate. Rats received intravenous (i.v.) glucose i...Continue Reading

References

Aug 1, 1991·The Journal of Clinical Investigation·M C MooreG I Shulman
Jun 1, 1989·Metabolism: Clinical and Experimental·I MagnussonB R Landau
May 1, 1988·The Journal of Clinical Investigation·H MiyoshiR R Wolfe
May 21, 1994·Journal of Theoretical Biology·R Rognstad
Nov 1, 1961·The Journal of Clinical Investigation·S Segal, A Blair

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Citations

Sep 9, 1999·Tissue Engineering·K LeeM L Yarmush
Aug 24, 1999·Current Opinion in Clinical Nutrition and Metabolic Care·L TappyM Berger
Nov 28, 2000·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·N PaquotL Tappy
Sep 23, 2000·American Journal of Physiology. Endocrinology and Metabolism·M DirlewangerL Tappy
Mar 10, 1999·The American Journal of Physiology·K F PetersenG I Shulman

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