Hepatic glutamine metabolism during endotoxemia in neonatal rats

Nutrition
Paisarn VejchapipatAgostino Pierro

Abstract

The liver plays a central role during endotoxemia. We investigated the biochemical changes that occur in neonatal liver during early stages of endotoxemia. Twenty neonatal rats (10 to 15 d; n = 10/group) were studied. Endotoxemic rats received intraperitoneal injections of 300 microg/kg of 12.5 mg/L of lipopolysaccharide and control rats received isovolemic normal saline. Two hours after injection, all lipopolysaccharide-injected animals exhibited signs of endotoxemia. Livers were removed and extracted into 12% perchloric acid. 1H and 31P magnetic resonance spectroscopy measured hepatic levels of glutamine, glutamate, alanine, lactate, glucose, beta-hydroxybutyrate, adenosine triphosphate, and adenosine diphosphate. Unpaired t test compared groups. No mortality occurred during the first 2 h after injection. Endotoxemia significantly decreased hepatic levels of glutamine (P < 0.001), glucose (P = 0.047), and beta-hydroxybutyrate (P < 0.001). There was no difference in hepatic levels of glutamate (P = 0.050), alanine (P = 0.165), lactate (P = 0.478), adenosine triphosphate (P = 0.165), and adenosine diphosphate (P = 0.136) between groups. Early endotoxemia caused significant changes in the hepatic metabolism of glutamine, glucose...Continue Reading

References

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Citations

Jun 5, 2002·Nutrition·Patrick A Ball, Gil Hardy
Dec 25, 2003·The Proceedings of the Nutrition Society·Simon Eaton
Jul 29, 2003·Current Opinion in Critical Care·Jan Wernerman
Apr 16, 2013·Antioxidants & Redox Signaling·Sarathi ManiRui Wang

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