Hepatic glycogen synthesis in farmed European seabass (Dicentrarchus labrax L.) is dominated by indirect pathway fluxes

Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
Ivan ViegasJohn G Jones

Abstract

Hepatic glycogen synthesis fluxes from direct and indirect pathways were quantified in seabass by postmortem (2)H NMR analysis of plasma water (PW) and glycogen glucosyl (2)H enrichments from (2)H-enriched seawater. Eighteen fish (28.0 ± 1.7 cm and 218.0 ± 43.0 g) were divided into three groups of 6 and studied over 24 days with transfer to 5% (2)H-seawater after day 21. Over this period, one group was fed daily with fishmeal, a second group was fasted, and a third group was fasted for 21 days followed by 3 days refeeding. Glycogen turnover and sources were determined from the ratio of glucosyl position 5 enrichment to that of plasma water (H5/PW). Glycogen levels of fed fish were significantly higher than fasted (665.4 ± 345.2 μmol.g(-1) liver versus 77.2 ± 59.5 μmol.g(-1) liver, P<0.05) while refed fish had comparable levels to fed (584.6 ± 140.4 μmol.g(-1) liver). Glycogen enrichment of fed fish was undetectable indicating negligible turnover over 3 days. For fasted fish, H5/PW was ~50% indicating that half of the glycogen had turned over via indirect pathway flux. For refed fish, H5/PW was ~100% indicating that the indirect pathway accounted for all net glycogen synthesis. Direct pathway conversion of dietary carbohydrate t...Continue Reading

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Citations

Aug 31, 2013·Fish Physiology and Biochemistry·Nicholas M WadeBrett D Glencross
Apr 22, 2014·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Ivan ViegasIsabel V Baanante
Jul 11, 2013·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Fátima O MartinsJohn G Jones
Jul 23, 2013·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Ivan ViegasJohn G Jones
May 30, 2017·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·João RitoJohn G Jones
Jul 31, 2018·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Ivana JarakJohn G Jones
Mar 20, 2020·The British Journal of Nutrition·N M WadeB D Glencross

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