Hepatic lipid peroxidation and cytochrome P-450 2E1 in pediatric nonalcoholic fatty liver disease and its subtypes

Journal of Clinical Gastroenterology
Lauren N BellNaga Chalasani

Abstract

To compare hepatic lipid peroxidation and cytochrome P-450 2E1 (CYP2E1) protein content in liver biopsies from children with nonalcoholic fatty liver disease (NAFLD) and 2 control groups. Elevated hepatic lipid peroxidation resulting from increased hepatic CYP2E1 enzyme activity is involved in the pathogenesis of NAFLD and nonalcoholic steatohepatitis (NASH) in adults, but studies in children are lacking. Liver biopsies from 59 children with NAFLD (49 with NASH), 10 children with normal liver histology, and 9 children with mild chronic hepatitis C (HCV) infection were examined. Hepatic malondialdehyde (a measure of lipid peroxidation) levels and CYP2E1 protein content were quantitated, as a percentage of the total area, by immunohistochemical staining of liver biopsy material followed by digital image quantitation. Lipid peroxidation was significantly greater in NAFLD liver biopsies (46.7 ± 20.8%) compared with biopsies from children with normal liver histology (7.6 ± 9.4%; P<0.001) or HCV infection (7.7 ± 7.6%; P<0.001). However, hepatic CYP2E1 expression was not different across the NAFLD, normal liver histology, and HCV groups (60.7 ± 8.7%, 53.5 ± 10.7%, and 60.0 ± 11.9%, respectively; P=0.116). Among children with NAFLD, li...Continue Reading

Citations

Sep 14, 2013·Liver International : Official Journal of the International Association for the Study of the Liver·Sonal DesaiLixin Zhu
Apr 4, 2012·Free Radical Research·Mayuko MoritaEtsuo Niki
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Sep 19, 2009·Contemporary Clinical Trials·Joel E LavineNonalcoholic Steatohepatitis Clinical Research Network Research Group
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Related Concepts

Teens
Biopsy
Steatohepatitis
Liver
Sodium Malondialdehyde
Retrospective Studies
Lipid Peroxidation
Body Mass Index Procedure
Nested Case-Control Studies
Cytochrome P-450 CYP2E1

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