PMID: 2505669Sep 1, 1989Paper

Hepatic lipoate uptake

Archives of Biochemistry and Biophysics
J PeinadoT P Akerboom

Abstract

Uptake of [35S]lipoate was studied in perfused rat liver and in isolated rat hepatocytes. During single-pass perfusion of [35S]lipoate about 30% of the radioactivity is retained in the liver. A substantial amount of 5,5'-dithiobis(2-nitrobenzoic acid)-reactive material appears in the effluent perfusate, while hepatic efflux of GSH is unchanged. The hepatic uptake of lipoate, the release of thiols, and also the biliary excretion of 35S-labeled compounds are suppressed by octanoate. In isolated hepatocytes the uptake of lipoate follows saturation kinetics showing a Km value of 38 microM and a Vmax of 180 pmol/mg X 10 s. The uptake is temperature-dependent; from the Arrhenius plot an activation energy of 14.8 kcal/mol at 20 microM lipoate is calculated. At high concentrations of lipoate (above 75 microM) a nonsaturable uptake component becomes predominant. Lipoate uptake is selectively inhibited by medium-chain fatty acids. Only slight inhibition is seen in the presence of long-chain fatty acids, and there is no inhibition with acetate or lactate. Substantial inhibition is also observed with acetylsalicylic acid, but not with taurocholate, bromosulfophthalein or biotin. Lipoate uptake can be inhibited by high concentrations of phl...Continue Reading

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Citations

Jul 17, 1995·Biochemical Pharmacology·A ConstantinescuL Packer
Apr 1, 1995·Free Radical Biology & Medicine·I MaitraL Packer
Aug 1, 1995·Free Radical Biology & Medicine·L PackerH J Tritschler
Jan 1, 1997·Free Radical Biology & Medicine·N HaramakiL Packer
Jun 2, 1998·Free Radical Biology & Medicine·J BustamanteB H Rihn
Nov 24, 1999·Free Radical Biology & Medicine·H Sies
Aug 27, 2014·The Veterinary Clinics of North America. Exotic Animal Practice·Johanna Mejia-Fava, Carmen M H Colitz
May 1, 1992·Toxicology and Applied Pharmacology·Z GregusC D Klaassen
Aug 4, 2015·Canadian Journal of Physiology and Pharmacology·Anjeza PashajRégis Moreau
Jan 1, 1992·Free Radical Research Communications·E SerbinovaL Packer
Feb 11, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·T M HagenA B Ames
Apr 3, 2004·Journal of Animal Physiology and Animal Nutrition·A S HillM M Christopher

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