PMID: 3214170Dec 1, 1988Paper

Hepatic mitochondrial coumarin 7-hydroxylase: comparison with the microsomal enzyme

Archives of Biochemistry and Biophysics
P HonkakoskiM A Lang

Abstract

The specific activity of cytochrome P450-linked coumarin 7-hydroxylase (COH) of hepatic mitoplasts from DBA/2N mice is up to 55% as great as the microsomal activity. According to Western blot and immunodiffusion analysis and inhibition studies with anti-P450Coh and metyrapone, the mitoplastic P450Coh had the same molecular weight and immunochemical and catalytic properties as the corresponding microsomal enzyme. The inducibility of the two proteins by pyrazole and their genetic regulation, as studied with DBA/2N and AKR/J mice, appears to be similar. However, the mitochondrial electron transfer system was not able to support the COH activity of reconstituted microsomal P450Coh although the enzyme was fully active with the microsomal NADPH-cytochrome P450 reductase. This indicates some differences between the two proteins with respect to their interaction with the electron transfer system. This was confirmed by the ability of anti-adrenodoxin reductase antibody to effectively inhibit the mitoplastic COH but not the COH reconstituted with purified microsomal P450Coh and NADPH-P450 reductase. We have previously found that P450Coh does not react with anti-P450b or anti-P450c antibodies, which recognize respective isoforms in rat li...Continue Reading

References

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Citations

May 26, 1993·Molecular and Cellular Biochemistry·M MagnaniG Gazzanelli
Oct 2, 2002·Journal of Neurochemistry·Sharon MiksysRachel F Tyndale
Jan 14, 2010·Journal of Biochemistry·Tsuneo Omura
Jan 25, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·S VisoniL F R Pinto
Dec 6, 2014·Toxicology and Applied Pharmacology·Siti Nur Fadzilah MuhsainA'edah Abu-Bakar
Aug 4, 2006·Chemico-biological Interactions·Tsuneo Omura
Mar 25, 2017·Journal of Biochemistry·Tsuneo Omura, Osamu Gotoh
Nov 1, 1989·Archives of Biochemistry and Biophysics·D W Rosenberg, A Kappas
Jan 1, 1990·Critical Reviews in Biochemistry and Molecular Biology·F P Guengerich
Oct 14, 2005·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Anna FranzénEva B Brittebo
Jan 1, 1994·Critical Reviews in Toxicology·M Pasanen, O Pelkonen

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