PMID: 1273Dec 15, 1975

Hepatic nucleases. Extrahepatic origin and association of neutral liver ribonuclease with lysosomes

European Journal of Biochemistry
J BartholeynsP Baudhuin

Abstract

In the large granule fraction of rat liver, the density distribution of inhibitor-sensitive neutral ribonuclease is similar to that for acid hydrolases and its density distribution is similarly modified by Triton WR-1339 accumulation in lysosomes. Particulate neutral ribonuclease is latent; the enzyme is unmasked by very low digitonin concentrations or hypoosmotic shock. These observations demonstrate that the bulk of liver neutral ribonuclease is associated with the lysosomal system. In view of the neutral pH optimum of the enzyme and of some particularities of its distribution in fractionation experiments, the possiblilty of an extrahepatic origin of neutral ribonuclease has been investigated. After partial pancreatectomy, a significant decrease is observed in both plasma and liver neutral ribonuclease. The effect is specific, for it does not occur for other lysosomal enzymes. Also, labelled bovine pancreatic ribonuclease, when injected intravenously, is taken up by the liver. The sedimentable labelled enzyme has a density distribution similar to the distribution of other foreign proteins, horseradish peroxidase or yeast invertase. These results are explained by the uptake of plasmatic neutral ribonuclease from pancreatic ori...Continue Reading

References

Sep 1, 1975·European Journal of Biochemistry·J BartholeynsP Baudhuin
Nov 1, 1967·The Biochemical Journal·M A Verity, A Reith
Nov 15, 1972·Biochemical and Biophysical Research Communications·S Hickman, E F Neufeld
Mar 21, 1974·Biochimica Et Biophysica Acta·M Zan-KowalczewskaD Shugar
Jan 1, 1974·International Journal of Peptide and Protein Research·J BatholeynsW H Stein
Sep 15, 1974·Biochemical Pharmacology·C De DuveF Van Hoof
May 22, 1969·Biochemical and Biophysical Research Communications·P BaudhuinC De Duve
Sep 4, 1959·Annals of the New York Academy of Sciences·E REID, J T NODES
Mar 1, 1962·Archives of Biochemistry and Biophysics·R L METZENBERG

Citations

Dec 1, 1987·Neurochemical Research·B AllinquantE Schuller
Jan 15, 1986·Clinica Chimica Acta; International Journal of Clinical Chemistry·B CzajkowskaJ Sznajd
Feb 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J Bartholeyns, P Baudhuin
Aug 1, 1978·The Journal of Cell Biology·R WattiauxF Dubois
May 23, 2007·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Gordana KocicSvetlana Stojanovic
Jun 1, 1982·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section C, Immunology·S AhoE Kulonen

Related Concepts

Cell Fractionation
Centrifugation, Density Gradient
Hydrogen-Ion Concentration
Liver
Lysosomes
Pancreatectomy
Vigilon
Plasma Protein Binding Capacity
Alkaline Ribonuclease
Subcellular Fractions

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