Hepatic oxidative stress, genotoxicity and vascular dysfunction in lean or obese Zucker rats

PloS One
Mille LøhrPeter Møller

Abstract

Metabolic syndrome is associated with increased risk of cardiovascular disease, which could be related to oxidative stress. Here, we investigated the associations between hepatic oxidative stress and vascular function in pressurized mesenteric arteries from lean and obese Zucker rats at 14, 24 and 37 weeks of age. Obese Zucker rats had more hepatic fat accumulation than their lean counterparts. Nevertheless, the obese rats had unaltered age-related level of hepatic oxidatively damaged DNA in terms of formamidopyrimidine DNA glycosylase (FPG) or human oxoguanine DNA glycosylase (hOGG1) sensitive sites as measured by the comet assay. There were decreasing levels of oxidatively damaged DNA with age in the liver of lean rats, which occurred concurrently with increased expression of Ogg1. The 37 week old lean rats also had higher expression level of Hmox1 and elevated levels of DNA strand breaks in the liver. Still, both strain of rats had increased protein level of HMOX-1 in the liver at 37 weeks. The external and lumen diameters of mesenteric arteries increased with age in obese Zucker rats with no change in media cross-sectional area, indicating outward re-modelling without hypertrophy of the vascular wall. There was increased ma...Continue Reading

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Citations

Nov 24, 2017·Frontiers in Pharmacology·Stefania SchiavoneLuigia Trabace
Oct 11, 2017·Mutagenesis·Peter MøllerSteffen Loft
Apr 6, 2020·Toxicology Letters·Andrew CollinsAmaya Azqueta

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Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

Image
GraphPad Prism

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