Hepatic progenitor cells from adult human livers for cell transplantation

Gut
T S WeissW E Thasler

Abstract

Liver regeneration is mainly based on cellular self-renewal including progenitor cells. Efforts have been made to harness this potential for cell transplantation, but shortage of hepatocytes and premature differentiated progenitor cells from extra-hepatic organs are limiting factors. Histological studies implied that resident cells in adult liver can proliferate, have bipotential character and may be a suitable source for cell transplantation. Particular cell populations were isolated after adequate tissue dissociation. Single cell suspensions were purified by Thy-1 positivity selection, characterised in vitro and transplanted in immunodeficient Pfp/Rag2 mice. Thy-1(+) cells that are mainly found in the portal tract and the surrounding parenchyma, were isolated from surgical liver tissue with high yields from specimens with histological signs of regeneration. Thy-1(+) cell populations were positive for progenitor (CD34, c-kit, CK14, M2PK, OV6), biliary (CK19) and hepatic (HepPar1) markers revealing their progenitor as well as hepatic and biliary nature. The potential of Thy-1(+) cells for differentiation in vitro was demonstrated by increased mRNA and protein expression for hepatic (CK18, HepPar1) and biliary (CK7) markers duri...Continue Reading

Citations

Apr 5, 2011·Journal of Materials Science. Materials in Medicine·Andreas K NusslerThomas S Weiss
Feb 8, 2011·In Vitro Cellular & Developmental Biology. Animal·Thomas J CapernaNeil C Talbot
Nov 4, 2011·Biological Trace Element Research·Yu DengYangang Wei
Dec 29, 2010·Stem Cells and Development·Jacobo Trebol LopezDamián García-Olmo
Feb 3, 2012·Respirology : Official Journal of the Asian Pacific Society of Respirology·Hiroshi Kubo
May 3, 2013·The Journal of Clinical Investigation·Taro Yamashita, Xin Wei Wang
Dec 9, 2008·Current Opinion in Organ Transplantation·Dhivya HaridassMichael Ott
Nov 30, 2011·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Jie Liu, Andreas Terzis
May 16, 2015·Molecular Medicine Reports·Yun HeYang Bi
Sep 19, 2009·Blood Cells, Molecules & Diseases·Selcuk SozerRonald Hoffman
May 1, 2009·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·E GaudioD Alvaro
Nov 19, 2011·Liver International : Official Journal of the International Association for the Study of the Liver·Laia TolosaM José Gómez-Lechón
Feb 18, 2014·Journal of Hepatology·Guido CarpinoEugenio Gaudio
Jun 15, 2011·Cell Transplantation·Gesine PlessUrsula Rauen
Jun 3, 2017·The International Journal of Artificial Organs·Elisa BelaschkMartina T Mogl
Oct 24, 2018·Nature Communications·Sonya A MacParlandIan D McGilvray
Aug 10, 2013·Cell Medicine·Catherine A LombardEtienne M Sokal
Oct 7, 2021·Transplant International : Official Journal of the European Society for Organ Transplantation·Hasan Al RezaTakanori Takebe

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