Hepatic stellate cells may be potential effectors of platelet activating factor induced portal hypertension

World Journal of Gastroenterology : WJG
Yan ChenYong-Ping Yang

Abstract

To determine platelet activating factor (PAF) receptor expression in cirrhotic hepatic stellate cells. Hepatic stellate cells, isolated from the livers of control and CCl(4)-induced cirrhotic rats, were placed in serum-free medium after overnight culture. We determined the PAF receptor in hepatic stellate cells by saturation binding technique and semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR), and the effects of PAF and its antagonist BN52021 on prostaglandin E(2) (PGE(2)) release by stellate cells. Scatchard analysis indicated the presence of PAF receptor with dissociation constant (Kd) of 4.66 nmol/L and maximum binding capacity (Bmax) of 24.65 fmol/microg in cirrhotic stellate cells. Compared with the control, the maximum PAF binding capacity increased significantly (Bmax: 24.65 +/- 1.96 fmol/microg. DNA, R = 0.982 vs 5.74 +/- 1.55 fmol/microg. DNA, R = 0.93; P < 0.01), whereas receptor affinity had no significant difference (Kd of 4.66 +/- 0.33 nmol/L for the cirrhosis and 3.51 +/- 0.26 nmol/L for the control; P > 0.05). Consistent with the receptor binding data, the mRNA expression of PAF receptor was increased significantly in cirrhotic stellate cells. PAF in a concentration-dependent manner ind...Continue Reading

References

Mar 1, 1991·The American Journal of Physiology·K L HinesR A Fisher
Sep 1, 1990·American Journal of Respiratory Cell and Molecular Biology·J GomezM Halonen
Aug 1, 1990·The American Journal of Physiology·M HalonenL M McManus
Mar 25, 1983·Nucleic Acids Research·U NudelD Yaffe
Apr 1, 1994·European Journal of Biochemistry·H BitoT Shimizu
Jun 15, 1993·The Biochemical Journal·W Chao, M S Olson
Jan 1, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D Rockey
Apr 6, 2000·American Journal of Physiology. Heart and Circulatory Physiology·B O IbeJ U Raj
Aug 31, 2000·Annual Review of Biochemistry·S M PrescottT M McIntyre
Oct 4, 2000·Physiological Reviews·G MontrucchioG Camussi
Dec 6, 2001·Experimental and Molecular Pathology·S W Luckey, D R Petersen
Jan 20, 2006·Liver International : Official Journal of the International Association for the Study of the Liver·A D GrypiotiM G Mykoniatis

❮ Previous
Next ❯

Citations

Apr 22, 2005·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·G CarpinoE Gaudio
Mar 24, 2004·Trends in Microbiology·Jean-Michel Pawlotsky
Mar 21, 2003·Biochemical and Biophysical Research Communications·Jude A ObenAnna Mae Diehl
Sep 9, 2000·Molecular Aspects of Medicine·G Poli
Mar 29, 2003·Journal of Hepatology·Kim R BridleGrant A Ramm
Dec 2, 1998·Journal of Hepatology·E Olaso, S L Friedman
Jan 4, 2001·The International Journal of Biochemistry & Cell Biology·D T DangV W Yang
Jun 28, 2003·Best Practice & Research. Clinical Gastroenterology·Linda M FletcherDarrell H G Crawford
Jul 31, 2018·Toxicological Research·Yoon Seok RohBumseok Kim

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.