Hepatitis B surface antibody purification with hepatitis B surface antibody imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-L-tyrosine methyl ester) particles

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Lokman UzunAdil Denizli

Abstract

Hepatitis B surface antibody imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-L-tyrosine methyl ester) particles were prepared for the purification of hepatitis B surface antibody from human plasma. N-methacryloyl-L-tyrosine methyl ester was chosen as a complexing agent for hepatitis B surface antibodies. Hepatitis B surface antibody imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-L-tyrosine methyl ester) particles were characterized by surface area measurements, swelling test, scanning electron microscopy, elemental analysis, and Fourier transform infrared spectroscopy. Ethylene glycol (1.0M) was used as desorption agent. Adsorption studies were performed from hepatitis B surface antibody and anti-hepatitis A antibody positive human plasma. Effects of antibody concentration, contact time, N-methacryloyl-L-tyrosine methyl ester content and temperature on the adsorption capacity were investigated. The amount of hepatitis B surface antibody adsorbed per unit mass increased with increasing hepatitis B surface antibody concentration, then reached saturation. Maximum hepatitis B surface antibody adsorption amount was 21.4 mIU/mg. Adsorption process reached the equilibrium in 60 min. Competitive adsorption of hepatitis...Continue Reading

References

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Mar 26, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Sergio da Silva e MoutaMarcia Terezinha Baroni de Moraes
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Apr 9, 2008·Journal of Chromatography. a·Nilay BereliAdil Denizli

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Citations

May 2, 2014·Applied Biochemistry and Biotechnology·Gizem ErtürkAdil Denizli
Mar 3, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Sevgi AsliyuceAdil Denizli
May 8, 2013·Biotechnology Journal·Telma BarrosoAna Aguiar-Ricardo
Apr 5, 2014·Journal of Molecular Recognition : JMR·Michael J WhitcombeIan A Nicholls
Aug 2, 2011·Biosensors & Bioelectronics·Gizem ErtürkAdil Denizli
Jan 29, 2011·Colloids and Surfaces. B, Biointerfaces·Müfrettin Murat SarıAdil Denizli
Aug 10, 2013·Colloids and Surfaces. B, Biointerfaces·Lokman UzunAdil Denizli
Dec 3, 2014·Colloids and Surfaces. B, Biointerfaces·Mehmet Emin ÇormanAdil Denizli
Mar 16, 2019·Sensors·Yeşeren SaylanAdil Denizli
May 30, 2019·Biomedical Chromatography : BMC·Sevgi AslıyüceAdil Denizli
Oct 17, 2012·Biomedical Chromatography : BMC·Nilay BereliAdil Denizli
May 24, 2021·Environmental Science and Pollution Research International·Kazım KöseLokman Uzun
May 18, 2021·The Analyst·Ghazaleh Jamalipour SoufiRajender S Varma
Aug 10, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Rekha JalandraPratima R Solanki

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