Hepatitis B virus core protein promotes the expression of neuraminidase 1 to facilitate hepatocarcinogenesis.

Laboratory Investigation; a Journal of Technical Methods and Pathology
Fanyun KongRenxian Tang

Abstract

Neuraminidase 1 (NEU1) has been reported to be associated with hepatocellular carcinoma (HCC). However, the function and associated molecular mechanisms of NEU1 in hepatitis B virus (HBV)-related HCC have not been well investigated. In the present study, the expression of NEU1 mediated by HBV and HBV core protein (HBc) was measured in hepatoma cells. The expression of NEU1 protein was detected via immunohistochemical analysis in HBV-associated HCC tissues. The role of NEU1 in the activation of signaling pathways and epithelial-mesenchymal transition (EMT) and the proliferation and migration of hepatoma cells mediated by HBc was assessed. We found that NEU1 was upregulated in HBV-positive hepatoma cells and HBV-related HCC tissues. HBV promoted NEU1 expression at the mRNA and protein level via HBc in hepatoma cells. Mechanistically, HBc was able to enhance the activity of the NEU1 promoter through NF-κB binding sites. In addition, through the increase in NEU1 expression, HBc contributed to activation of downstream signaling pathways and EMT in hepatoma cells. Moreover, NEU1 facilitated the proliferation and migration of hepatoma cells mediated by HBc. Taken together, our findings provide novel insight into the molecular mechanis...Continue Reading

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Citations

Nov 17, 2020·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Fanyun KongRenxian Tang

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Datasets Mentioned

BETA
GSE14520

Methods Mentioned

BETA
transfection
PCR
electrophoresis

Software Mentioned

Affymetrix Transcriptome Analysis Console
Affymetrix Expression Console
ImageJ
TFSEARCH
SPSS

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