Hepatitis B virus DNA polymerase gene polymorphism based prediction of genotypes in chronic HBV patients from Western India

African Health Sciences
Yashwant G ChavanRabindra N Misra

Abstract

Hepatitis B Virus (HBV) infection is one of the major causes of liver cirrhosis, hepatocellular carcinoma and deaths due to the acute or chronic consequences worldwide. HBV is distributed into various genotypes based on nucleic acid sequence variation. To develop a method of HBV genotyping and drug resistance interpretation using partial sequencing of polymerase gene. This study was performed on 98 HBV infected patients' serum samples from Western India. A nested PCR protocol was designed for amplification of pol gene from HBV genome and Sanger's sequencing of the gene fragment. Sequences were aligned with HBV reference sequences for phylogenetic analysis and for characterization of genetic diversity. Drug resistance mutations were screened using HBVSeq program from Stanford University. Distribution of HBV genotypes showed predominance of genotype D, circulating in 76 (77.55%) patients (p < 0.05). Genotypes A and C were less prevalent and were identified in 4 (4.08%) and 18 (18.37%) patients, respectively. Anti-retroviral drug resistance mutations were not detected in any patient. A method for determination of HBV genotypes using pol gene sequencing which simultaneously detects major drug resistance mutations has been establish...Continue Reading

Citations

Jun 2, 2018·Indian Journal of Gastroenterology : Official Journal of the Indian Society of Gastroenterology·Brij SharmaRakesh Aggarwal
Dec 29, 2020·AIMS Microbiology·Manoj Kumar Rajput

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Datasets Mentioned

BETA
AB073838
AB036907
AY090457
KM076939-KM077016
AF151735
AB064313
AY090454
AB090270

Methods Mentioned

BETA
genotyping
PCR
electrophoresis

Software Mentioned

MEGA
Geno2pheno
Excel
SPSS
Primer
ClustalW
HBVSeq
Chromas Pro

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