Hepatitis B virus genome recycling and de novo secondary infection events maintain stable cccDNA levels.

Journal of Hepatology
Chunkyu KoUlrike Protzer

Abstract

Several steps in the HBV life cycle remain obscure because of a lack of robust in vitro infection models. These steps include particle entry, formation and maintenance of covalently closed circular (ccc) DNA, kinetics of gene expression and viral transmission routes. This study aimed to investigate infection kinetics and cccDNA dynamics during long-term culture. We selected a highly permissive HepG2-NTCP-K7 cell clone engineered to express sodium taurocholate co-transporting polypeptide (NTCP) that supports the full HBV life cycle. We characterized the replication kinetics and dynamics of HBV over six weeks of infection. HBV infection kinetics showed a slow infection process. Nuclear cccDNA was only detected 24 h post-infection and increased until 3 days post-infection (dpi). Viral RNAs increased from 3 dpi reaching a plateau at 6 dpi. HBV protein levels followed similar kinetics with HBx levels reaching a plateau first. cccDNA levels modestly increased throughout the 45-day study period with 5-12 copies per infected cell. Newly produced relaxed circular DNA within capsids was reimported into the nucleus and replenished the cccDNA pool. In addition to intracellular recycling of HBV genomes, secondary de novo infection events re...Continue Reading

Citations

Mar 29, 2019·Life Science Alliance·Peter Ac WingJane A McKeating
Mar 30, 2019·The Journal of Infectious Diseases·Jan-Hendrik BockmannUlrike Protzer
Sep 5, 2019·International Journal of Molecular Sciences·Nur K Mohd-IsmailYee-Joo Tan
Jul 7, 2019·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Yongzhen LiuFengmin Lu
Aug 4, 2019·Journal of Virology·Jianming HuAleem Siddiqui
Oct 30, 2019·Antimicrobial Agents and Chemotherapy·Chunkyu KoUlrike Protzer
Sep 24, 2020·International Journal of Molecular Sciences·Sergey BrezginVladimir Chulanov
Aug 18, 2020·Cellular Microbiology·Anindita ChakrabortyJane A McKeating
Dec 18, 2019·The Journal of General Virology·Valentina D'ArienzoJane A McKeating
May 1, 2019·The Journal of Clinical Investigation·Karin WisskirchenUlrike Protzer
Aug 6, 2020·Viruses·Jochen M Wettengel, Benjamin J Burwitz
Sep 19, 2020·Microorganisms·Magda Rybicka, Krzysztof Piotr Bielawski
Oct 3, 2020·Cellular Microbiology·Valentina D'ArienzoJoanna L Parish
Sep 25, 2020·Microbiology and Immunology·Nelly Gakii Muriungi, Keiji Ueda
Sep 23, 2020·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Shuya MatsudaNobuyuki Enomoto
Nov 6, 2020·Infection and Drug Resistance·Maura Dandri, Joerg Petersen
Dec 29, 2020·Journal of Extracellular Vesicles·Stephanie JungUlrike Protzer
May 8, 2020·Antiviral Research·Claire Pierra RouviereJohn E Tavis
Mar 19, 2021·Virus Evolution·Katrina A LythgoePhilippa C Matthews
Mar 14, 2021·Nature Communications·Xiaodong ZhuangJane A McKeating
Jan 14, 2020·Current Opinion in Systems Biology·Ashish GoyalAlan S Perelson
May 2, 2021·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Monique D AppelmanStan F J van de Graaf
May 30, 2021·Journal of Hepatology·Maria Guadalupe MartinezFabien Zoulim
Sep 1, 2020·Antiviral Research·Senko Tsukuda, Koichi Watashi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.