Hepatitis B virus small surface antigen particles are processed in a novel endosomal pathway for major histocompatibility complex class I-restricted epitope presentation

European Journal of Immunology
R SchirmbeckJ Reimann

Abstract

We investigated the major histocompatibility complex (MHC) class I-restricted presentation of an epitope of the hepatitis B virus small surface (S) antigen particle to cloned murine cytotoxic T lymphocytes (CTL). Efficient Ld-restricted presentation of the S28-39 epitope to CTL is observed in cells of different tissue origin pulsed in vitro, either with the antigenic S28-39 12-mer S-peptide, or with particulate S-antigen. The kinetics of epitope presentation differ in S-peptide-pulsed and in S-particle-pulsed cells: while a 15-min pulse with the antigenic peptide sensitizes targets for class I-restricted CTL lysis, presentation of S-particles requires 30-60 min to sensitize cells for CTL lysis. Uptake of antigenic material and active metabolism of the presenting cell are required for processing of S-particles, but not for sensitizing targets with S-peptides. Intracellular processing and presentation of S-particles is blocked in cells treated with chloroquine, NH4Cl, primaquine, or leupeptin, but not by treatment with cycloheximide or brefeldin A. This processing pathway operates efficiently in peptide-transporter-deficient, Ld-transfected T2 cells, revealing a novel endosomal/lysosomal processing pathway for class I-restricted ...Continue Reading

References

May 1, 1992·The Journal of Experimental Medicine·A FrancoV Barnaba
May 1, 1992·Immunology Today·J J Neefjes, H L Ploegh
Feb 15, 1992·Experientia·P O Seglen, P Bohley
Jan 1, 1991·Annual Review of Immunology·F M Brodsky, L E Guagliardi
Jan 1, 1989·Annual Review of Immunology·A Townsend, H Bodmer
Jan 1, 1987·Annual Review of Biochemistry·D Ganem, H E Varmus
Jan 1, 1984·The Journal of Experimental Medicine·D B Tse, B Pernis
Nov 1, 1993·The Journal of Experimental Medicine·K AndoF V Chisari
Jun 1, 1993·Current Biology : CB·M T Heemels, H Ploegh
Feb 1, 1985·Immunology Today·B Pernis

❮ Previous
Next ❯

Citations

Jan 1, 1997·Springer Seminars in Immunopathology·M Corr, H Tighe
Mar 1, 1996·Journal of Clinical Immunology·C V Harding
Aug 30, 2002·Molecular Immunology·Monique Grommé, Jacques Neefjes
Aug 26, 1998·Immunology Today·F R CarboneW R Heath
Jan 23, 1998·Peptides·A Maffei, P E Harris
May 1, 1997·Human Immunology·A MaffeiP E Harris
Apr 23, 2003·Microbes and Infection·Georg LautschamNeil Blake
Mar 15, 2001·Traffic·M J KleijmeerH J Geuze
Mar 22, 2002·Nature Reviews. Immunology·W R Heath, F R Carbone
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Roger Belizaire, Emil R Unanue
Jul 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·X S HeW S Robinson
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·M GromméJ Neefjes
Oct 1, 1996·The Journal of Experimental Medicine·M CorrH Tighe
Jan 13, 2000·Immunological Reviews·M J Wick, H G Ljunggren
Aug 3, 2013·The Journal of Clinical Investigation·Adam J GehringAntonio Bertoletti
Nov 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Stefan FreigangRolf M Zinkernagel
Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·G K BalendiranJ C Sacchettini
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·M RescignoP Ricciardi-Castagnoli

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.