Hepatitis B virus X gene expression is activated by X protein but repressed by p53 tumor suppressor gene product in the transient expression system

Virology
S TakadaK Koike

Abstract

Hepatitis B virus (HBV) X gene is known to exhibit a transcriptional activation function and is considered to play a major role in hepatocarcinogenesis. We determined a 20-bp promoter element for the HBV X gene transcription and found a binding protein to this promoter element, designated as an X-PBP. We then examined the effects of HBV X protein and p53 tumor suppressor gene product on X gene transcription from the 20-bp promoter element using the transient expression technique. Activity of the X gene promoter was stimulated by X protein expression, but, in contrast, was repressed by transfected normal p53 gene. On the other hand, mutant p53 gene product exhibited no repression. Moreover, the p53 repression of X gene transcription was canceled by X protein coexpression. Thus, the effects of X protein and normal p53 product appear to be mutually antagonistic in the regulation of X gene expression. However, mutated promoter elements which failed to bind to X-PBP still responded to X protein or p53, indicating that the process of X transactivation or p53 repression may be independent of X-PBP binding to the promoter element. Our data suggest that X protein could disrupt function of normal p53 protein in X gene-transfected cells.

Citations

Feb 5, 1999·Journal of Hepato-biliary-pancreatic Surgery·M A Feitelson
Sep 13, 2002·Journal of Viral Hepatitis·Naazneen MoollaPatrick Arbuthnot
Apr 6, 2006·Internal Medicine·Kiwamu Okita
Apr 1, 1997·Pathology International·T UchidaH Shinzawa
May 26, 2009·Cancer Letters·Katsuro Koike
Nov 15, 2000·Critical Reviews in Clinical Laboratory Sciences·C Rabe, W H Caselmann
Feb 17, 2006·Biology of the Cell·Lauréline RogerPierre Roux
May 4, 2017·International Journal of Molecular Sciences·Maria FerraiuoloSabrina Strano
Aug 29, 2006·World Journal of Gastroenterology : WJG·Nicola FiedlerStephan Schaefer
May 29, 2000·Scandinavian Journal of Infectious Diseases·A WidellS Wallerstedt

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