Hepatitis B virus X protein induces the histone H3 lysine 9 trimethylation on the promoter of p16 gene in hepatocarcinogenesis

Experimental and Molecular Pathology
Di-Yi WangRong Zhu

Abstract

Our previous study showed hepatitis B virus X protein (HBx) suppresses the p16 expression in hepatocarcinogenesis. In this study we explored the relationship between HBx and trimethylation of H3K9 (H3K9me3), and elucidated the underlying mechanisms in HBx inducing the tumor suppressor p16 gene silence. SMMC-7721 and HepG2 hepatoma cell lines were transfected with HBx-expressing plasmid. Immunohistochemistry, Western blotting and real-time polymerase chain reaction, were performed to detect the expressions of HBx, H3K9me3, and jumonji domain-containing protein 2B (JMJd2B). H3K9me3 enrichment on the p16 promoter was measured by immunoprecipitation-PCR (ChIP-PCR) analyses, and 39 cases of hepatitis B virus (HBV) associated-hepatocellular carcinoma (HCC) and corresponding noncancerous liver tissues were also examined. We demonstrated that HBx was able to upregulate H3K9me3 and suppress JMJd2B mRNA and protein levels in SMMC-7721 and HepG2 hepatoma cell lines. JMJd2B, as a specific target of H3K9me3 for demethylation, was inversely correlated with the levels of H3K9me3 in SMMC-7721 (r=-0.666, P<0.05) and HepG2 cells (r=-0.625, P<0.05). The ChIP-PCR data indicated that HBx remarkably increased H3K9me3 on the p16 promoter region. Immu...Continue Reading

References

Dec 17, 2002·Molecular and Cellular Biology·Yutaka KondoJean-Pierre J Issa
Feb 7, 2006·The Journal of Laboratory and Clinical Medicine·Xiaodong ZhangLihong Ye
Jun 2, 2006·Genes & Development·Barna D FodorThomas Jenuwein
Dec 26, 2006·Cancer Letters·Mark A Feitelson, Jungmin Lee
Mar 7, 2007·Nature Reviews. Genetics·Manel Esteller
Jun 16, 2007·Gastroenterology·Hashem B El-Serag, K Lenhard Rudolph
Feb 27, 2010·Journal of Hepatology·Christine NeuveutMarie Annick Buendia
Jul 8, 2010·International Journal of Cancer. Journal International Du Cancer·Soyoung LimReinhard Buettner
Apr 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Lei ShiYongfeng Shang
Nov 29, 2013·Archives of Pathology & Laboratory Medicine·Ronghui XiaJiang Li
May 23, 2014·International Journal of Cancer. Journal International Du Cancer·Tetsuhiro ChibaOsamu Yokosuka
Sep 23, 2014·The International Journal of Biochemistry & Cell Biology·Qiliang XiXuejun Zhang
Sep 28, 2014·Best Practice & Research. Clinical Gastroenterology·Cristina BosettiCarlo La Vecchia
Dec 17, 2014·Biochimica Et Biophysica Acta·Mario VenzaIsabella Venza

❮ Previous
Next ❯

Citations

Jan 18, 2017·Hepatology International·Ming WangQin Ning
Jan 22, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Dong Hoon LeeSo Hee Kwon
Aug 29, 2018·BioMed Research International·Xinghui LiuBinghong Zhang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.