PMID: 9525629Apr 3, 1998Paper

Hepatitis C virus core from two different genotypes has an oncogenic potential but is not sufficient for transforming primary rat embryo fibroblasts in cooperation with the H-ras oncogene

Journal of Virology
J ChangY C Sung

Abstract

Persistent infection with hepatitis C virus (HCV) is associated with the development of liver cirrhosis and hepatocellular carcinoma. To examine the oncogenic potential of the HCV core gene product, primary rat embryo fibroblasts (REFs) were transfected with the core gene in the presence or absence of the H-ras oncogene. In contrast to a previous report (R. B. Ray, L. M. Lagging, K. Meyer, and R. Ray, J. Virol. 70:4438-4443, 1996), HCV core proteins from two different genotypes (type 1a and type 1b) were not found to transform REFs to tumorigenic phenotype in cooperation with the H-ras oncogene, although the core protein was successfully expressed 20 days after transfection. In addition, REFs transfected with E1A- but not core-expressing plasmid showed the phenotype of immortalized cells when selected with G418. The biological activity was confirmed by observing the transcription activation from two viral promoters, Rous sarcoma virus long terminal repeat and simian virus 40 promoter, which are known to be activated by the core protein from HCV-1 isolate. In contrast to the result with primary cells, the Rat-1 cell line, stably expressing HCV core protein, exhibited focus formation, anchorage-independent growth, and tumor forma...Continue Reading

References

May 1, 1978·Journal of Virology·D R LowyE M Scolnick
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·V BandR Sager
Oct 1, 1990·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K KiyosawaR H Purcell
Jun 1, 1986·Molecular and Cellular Biology·H LandR A Weinberg
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R PollackD Rifkin
Oct 1, 1994·Journal of Clinical Gastroenterology·A M Di BisceglieJ H Hoofnagle
Aug 1, 1994·Japanese Journal of Medical Science & Biology·D W KimT Miyamura
Jun 1, 1995·Journal of Virology·D SakamuroT Takegami
Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·J BukhR H Miller
Jun 1, 1994·Journal of Virology·E SantoliniN La Monica
Feb 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·G BarbaC Bréchot
Mar 1, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C PasquinelliF V Chisari
Apr 1, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T KawamuraT J Liang
Apr 25, 1997·The Journal of Biological Chemistry·R B RayR Ray

❮ Previous
Next ❯

Citations

May 29, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M HondaS M Lemon
Mar 29, 2001·Journal of Clinical Gastroenterology·M Y ShapiraI Gotsman
Jan 5, 2002·Microbiology and Immunology·I YoshidaH Hotta
Mar 9, 2002·The Journal of Biological Chemistry·Agoritsa VarakliotiPenelope Mavromara
Jan 30, 2004·The Journal of Biological Chemistry·Kyoko Tsukiyama-KoharaMichinori Kohara
Dec 23, 2004·Journal of Virology·Ryosuke SuzukiTetsuro Suzuki
Apr 22, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Mark Branda, Jack R Wands
Oct 7, 2006·Current Opinion in Gastroenterology·G A Macdonald
Apr 16, 2009·Reviews in Medical Virology·Chad D Williamson, Anamaris M Colberg-Poley
Jun 22, 2000·The Journal of General Virology·R Bartenschlager, V Lohmann
Jul 12, 2002·Journal of Gastroenterology·Kunitada ShimotohnoMakoto Hijikata
Aug 20, 2002·The Journal of General Virology·Mi Nam LeeKyung Lib Jang
Aug 1, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·N KatoM Omata
Dec 22, 1999·Journal of Viral Hepatitis·J M Pawlotsky, G Germanidis
Nov 25, 2003·Journal of Medical Virology·Hiroyoshi TaniguchiMasao Omata
Sep 27, 2007·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Aldona Kasprzak, Agnieszka Adamek
Jan 3, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ahmed El-ShamyHak Hotta
Mar 19, 2020·Journal of Clinical Medicine·Ana Rita FerreiraDaniela Ribeiro
Jun 15, 2001·ILAR Journal·M A Feitelson, J D Larkin
Dec 3, 1999·European Journal of Clinical Investigation·F L DumoulinU Spengler
Apr 26, 2007·Folia Microbiologica·L KrekulováL W Riley
Apr 21, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Takayoshi FukutomiJisu Li
Dec 18, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Shirit EinavJeffrey S Glenn
May 8, 2003·Journal of Biosciences·Nicole Pavio, Michael M C Lai
Jun 10, 2004·World Journal of Gastroenterology : WJG·Min LiuQian Yang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.