Hepatitis C virus core protein binding to lipid membranes: the role of domains 1 and 2

Journal of Viral Hepatitis
Ana J Pérez-BernáJosé Villalaín

Abstract

We have analysed and identified different membrane-active regions of the Hepatitis C virus (HCV) core protein by observing the effect of 18-mer core-derived peptide libraries from two HCV strains on the integrity of different membrane model systems. In addition, we have studied the secondary structure of specific membrane-interacting peptides from the HCV core protein, both in aqueous solution and in the presence of model membrane systems. Our results show that the HCV core protein region comprising the C-terminus of domain 1 and the N-terminus of domain 2 seems to be the most active in membrane interaction, although a role in protein-protein interaction cannot be excluded. Significantly, the secondary structure of nearly all the assayed peptides changes in the presence of model membranes. These sequences most probably play a relevant part in the biological action of HCV in lipid interaction. Furthermore, these membranotropic regions could be envisaged as new possible targets, as inhibition of its interaction with the membrane could potentially lead to new vaccine strategies.

References

Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·T A ChaM S Urdea
Jul 7, 1981·Biochemistry·D K StruckR E Pagano
Oct 1, 1996·Nature Structural Biology·W C Wimley, S H White
Jun 1, 1997·The Journal of General Virology·O NolandtH G Kräusslich
Jul 20, 1999·Annual Review of Biophysics and Biomolecular Structure·S H White, W C Wimley
Mar 9, 2002·Journal of Virology·Anna AhnMargaret Kielian
Nov 5, 2002·Nature Reviews. Drug Discovery·Seng-Lai TanNahum Sonenberg
Sep 26, 2003·Biophysical Journal·Rodrigo F M de AlmeidaManuel Prieto
Jan 31, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·François PeninJean-Michel Pawlotsky
Dec 23, 2004·Journal of Virology·Ryosuke SuzukiTetsuro Suzuki
Aug 17, 2005·Journal of Virology·Steeve BoulantJean-Pierre Lavergne
Feb 18, 2006·Biochimica Et Biophysica Acta·Miguel R MorenoJosé Villalaín
Mar 11, 2006·The Journal of General Virology·Malika Ait-GoughoultePhilippe Roingeard
Mar 15, 2006·Biochemistry·Ana J Pérez-BernaJosé Villalaín
Apr 15, 2006·International Journal of Medical Sciences·Stephen L Chen, Timothy R Morgan
May 18, 2006·The Journal of Biological Chemistry·Steeve BoulantJohn McLauchlan
Jul 20, 2006·The Journal of Biological Chemistry·Christelle Vauloup-FellousArielle R Rosenberg
Oct 19, 2006·Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre·M Irshad, Indranil Dhar
Dec 5, 2006·Biochemical and Biophysical Research Communications·Sang Kyu LeeYoung Sang Kim

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Citations

Sep 27, 2012·FEBS Letters·Kirill TsirulnikovAlexander Pushkin
Aug 15, 2014·The Journal of Physical Chemistry. B·Henrique Nemésio, José Villalaín
Aug 25, 2009·Biochimica Et Biophysica Acta·Ana J Pérez-BernáJosé Villalaín
Jul 10, 2012·Biochimica Et Biophysica Acta·Henrique NemésioJosé Villalaín
Jul 23, 2011·Biochimica Et Biophysica Acta·Henrique NemésioJosé Villalaín
Jun 12, 2013·Molecular Membrane Biology·Henrique NemésioJosé Villalaín
Feb 5, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Saba KhaliqAsim Pervaiz
Nov 27, 2009·Virology Journal·Ashraf MohamadkhaniGhodratollah Montazeri
Feb 16, 2010·Biochimica Et Biophysica Acta·M Francisca Palomares-JerezJosé Villalaín
Jul 28, 2009·Biochimica Et Biophysica Acta·Jaime GuillénJosé Villalaín
Jul 31, 2014·Biochemistry·Henrique Nemésio, José Villalaín

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Methods Mentioned

BETA
size exclusion chromatography
infrared spectroscopy
circular dichroism

Software Mentioned

Spectra
Calc

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