Hepatitis C virus core region: helper T cell epitopes recognized by BALB/c and C57BL/6 mice

The Journal of General Virology
K KakimiM Seki

Abstract

In this study, we characterized the B cell and T cell responses to the hydrophilic portion of hepatitis C virus (HCV) core protein in two strains of mice and identified the respective antigen determinants. BALB/c (H-2d) and C57BL/6 (B6:H-2b) mice were immunized by a subcutaneous injection of recombinant HCV core protein together with Freund's complete adjuvant. The level of antibody production, as determined by ELISA, was consistently higher in BALB/c than in B6 mice. However, antibodies in sera from each strain bound to the N-terminal region of the core protein within amino acids 1 to 28 (MSTNPKPQRKIKRNTNRRPQDVKFPGGG), according to an experiment using non-overlapping peptides that covered the hydrophilic portion of HCV core protein. The T cell responses were also higher in BALB/c than in B6 mice with respect to the proliferative responses of the draining lymph node cells in vitro. By limiting dilution cultures of the draining lymph node cells in vitro repetitively stimulated with recombinant core protein, T cell clones were established from both strains of mice and characterized. The surface markers of these clones were Thy-1.2+, CD3+, TCR alpha beta+, CD4+ and CD8+. The proliferative responses were inhibited in the presence o...Continue Reading

Citations

Jan 1, 1996·Pathology Oncology Research : POR·Zsuzsa SchaffRolf Schulte-Herman
Nov 27, 2015·Journal of Immunology Research·Irina SominskayaMaria Isaguliants
Feb 26, 2005·Journal of Virology·Reimar JohneHermann Müller
Nov 26, 2009·Uirusu·Koki TaniguchiMasanori Kugita
Feb 9, 2016·Jundishapur Journal of Microbiology·Sara MohammadzadehAli Hatef Salmanian

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