Hepatitis C virus genotype-3a core protein enhances sterol regulatory element-binding protein-1 activity through the phosphoinositide 3-kinase-Akt-2 pathway

The Journal of General Virology
Candice Jackel-CramQiang Liu

Abstract

Hepatitis C virus genotype-3a (HCV-3a) is directly linked to the development of steatosis. We previously showed that, through sterol regulatory element binding protein-1 (SREBP-1), HCV-3a core protein upregulates the promoter activity of fatty acid synthase, a major enzyme involved in de novo lipid synthesis. In this study, we investigated whether HCV-3a core can activate SREBP-1 and studied the role of phosphoinositide 3-kinase (PI3K)-Akt-2 pathway in modulating SREBP-1 activity by HCV-3a core. To determine whether HCV-3a core could activate SREBP-1, the level of mature SREBP-1 was analysed by Western blotting. Our results showed that the level of mature SREBP-1 was enhanced by HCV-3a core protein after transient expression and in the chimeric HCV-3a core/1b replicon cells in comparison to controls. To investigate the role of the PI3K-Akt-2 pathway in SREBP-1 activation by HCV-3a core, PI3K and Akt-2 activity was inhibited by using the chemical inhibitor LY294002, a dominant-negative Akt-2 plasmid, or knockdown of Akt-2 by small hairpin RNA. Our results showed that inhibition of PI3K and Akt-2 was associated with reduced SREBP-1 activation by HCV-3a core. These results indicate a role for PI3K and Akt-2 in increasing SREBP-1 a...Continue Reading

References

Apr 1, 1973·Virology·F L Graham, A J van der Eb
Jul 22, 1998·Journal of Hepatology·P AmorosoL Sagliocca
Aug 26, 1998·Biochemical and Biophysical Research Communications·J KotzkaW Krone
Jul 25, 2000·Journal of Hepatology·L Rubbia-BrandtF Negro
Aug 1, 2000·The Journal of Biological Chemistry·G RothD Müller-Wieland
Mar 22, 2002·Journal of Virology·Thomas PietschmannRalf Bartenschlager
Oct 24, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Dinesh KumarJacob George
Feb 14, 2003·Journal of Virology·Volker LohmannRalf Bartenschlager
Jun 28, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Thierry PoynardJanice Albrecht
Dec 31, 2003·Lancet·Thierry PoynardChing Lung Lai
Jan 8, 2004·The Journal of Biological Chemistry·Andrew StreetMark Harris
May 8, 2004·Trends in Biochemical Sciences·Derek P BrazilBrian A Hemmings
Apr 14, 2005·Journal of Hepatology·Karim AbidFrancesco Negro
Dec 31, 2005·American Journal of Physiology. Cell Physiology·Min Lu, John Y-J Shyy
May 3, 2006·Proceedings of the National Academy of Sciences of the United States of America·Thomas PietschmannRalf Bartenschlager
Jul 19, 2006·Nature Reviews. Genetics·Jeffrey A EngelmanLewis C Cantley
Nov 16, 2006·World Journal of Gastroenterology : WJG·Francesco Negro
Feb 17, 2007·Current Opinion in Cell Biology·Maria T Bengoechea-Alonso, Johan Ericsson
Feb 23, 2008·Trends in Endocrinology and Metabolism : TEM·Rajendra RaghowMarshall B Elam
May 1, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Muhammad Y SheikhArun J Sanyal
Jun 24, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Aurélie PiodiJean-Michel Pawlotsky
Aug 30, 2008·Current Opinion in Pharmacology·Timothy A YapPaul Workman
Sep 23, 2008·Liver International : Official Journal of the International Association for the Study of the Liver·Valerio PazienzaFrancesco Negro
Jan 8, 2009·The Journal of Biological Chemistry·Maria T Bengoechea-Alonso, Johan Ericsson
Jul 25, 2009·Nature Reviews. Cancer·Jeffrey A Engelman

❮ Previous
Next ❯

Citations

Mar 1, 2012·Histochemistry and Cell Biology·Stefan Hübner, Athina Efthymiadis
Jul 25, 2013·The International Journal of Biochemistry & Cell Biology·Wei LiuHuijun Duan
Feb 21, 2013·Biochemical and Biophysical Research Communications·Ling QiaoQiang Liu
May 26, 2012·Antiviral Research·Matthieu BlanchetPatrick Labonté
Apr 11, 2012·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Qi Wu, Qiang Liu
Apr 6, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Sophie ClémentMichelangelo Foti
Jan 11, 2012·Liver International : Official Journal of the International Association for the Study of the Liver·Shiv K Sarin, Chandan K N Kumar
Aug 15, 2015·Medical Microbiology and Immunology·Ketti G OliveiraJoão R R Pinho
Dec 19, 2013·Journal of Cellular Physiology·Jun HaoHuijun Duan
Jul 31, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ragunath SingaraveluJohn Paul Pezacki
Mar 25, 2015·Biochemical and Biophysical Research Communications·Qi WuQiang Liu
Aug 4, 2015·Biochemical and Biophysical Research Communications·Brett HoffmanQiang Liu
Mar 13, 2014·Antiviral Research·Ben VerpaalenLeen Delang
Mar 13, 2015·ACS Infectious Diseases·Ragunath SingaraveluJohn Paul Pezacki
Feb 5, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Saba KhaliqAsim Pervaiz
Oct 28, 2016·Hepatology International·Sarah KattakuzhyShyam Kottilil
Apr 20, 2017·Current Infectious Disease Reports·Steven W JohnsonBrianne Raccor
May 30, 2012·Laboratory Investigation; a Journal of Technical Methods and Pathology·María Victoria García-MediavillaSonia Sánchez-Campos
Jun 18, 2017·Scientific Reports·Qi WuQiang Liu
Jan 12, 2017·Drugs·Austin ChanSusanna Naggie
Jan 13, 2018·Scientific Reports·Ragunath SingaraveluJohn P Pezacki
Apr 12, 2011·Expert Review of Gastroenterology & Hepatology·Venessa PattulloJacob George
Oct 3, 2018·Journal of Clinical and Translational Hepatology·Azam Moslehi, Zeinab Hamidi-Zad
Feb 6, 2018·Biochemical and Biophysical Research Communications·Zhubing Li, Qiang Liu
Mar 30, 2021·World Journal of Gastroenterology : WJG·Dorota Zarębska-Michaluk
Aug 2, 2021·Microbial Pathogenesis·S T R MoolamallaP K Vinod

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.