PMID: 9431953Feb 12, 1998Paper

Hepatitis C virus heteroduplex tracking assay for genotype determination reveals diverging genotype 2 isolates in Italian hemodialysis patients

Journal of Clinical Microbiology
P L CalvoA J Weiner

Abstract

A heteroduplex tracking assay (HTA) was developed for genetic analyses of the hepatitis C virus (HCV) using single-stranded probes from the core (C)/E1 region. Nucleotide sequencing of reverse transcriptase (RT)-PCR products from 15 Italian dialysis patients confirmed the specificity and accuracy of the HTA genotyping method, which identified 5 of 15 (33.3%) 1b, 7 of 15 (46.7%) 3a, and 3 of 15 (20%) type 2 infections. The genotypes of an additional 12 HCV antibody-positive blood donors from different geographical locations were also in agreement with the genotypes determined by the Inno-LiPA HCV II kit (Innogenetics) and/or restriction fragment length polymorphism (RFLP). Isolates which had between 35 to 40% nucleotide divergence from control subtype 1a, 1b, 2a, 2b, or 3a standards could be typed. Surprisingly, HTA detected one 1b-2 coinfection which was missed by DNA sequencing. Three samples that were designated non-2a or 2b type 2 by HTA were found to be type 2a by both RFLP and direct nucleotide sequencing of the 5' untranslated region. The genetic distance between patient type 2 and control 2a, 2b, and 2c isolates indicated that a new subtype was present in the population being studied. Serotyping (RIBA serotyping strip im...Continue Reading

References

May 1, 1992·The Journal of General Virology·S W ChanP Simmonds
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·Y K ShimizuH Yoshikura
Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J H HanM Houghton
Feb 28, 1991·Biochemical and Biophysical Research Communications·M HijikataK Shimotohno
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·Q L ChooP J Barr
Aug 16, 1990·Biochemical and Biophysical Research Communications·N EnomotoT Date
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·N KatoK Shimotohno
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·L StuyverG Maertens
Feb 1, 1995·Seminars in Liver Disease·J BukhR H Purcell
Feb 1, 1995·Seminars in Liver Disease·M J Alter
Jul 1, 1994·The Journal of General Virology·M SakamotoH Okamoto
Oct 1, 1995·Journal of Clinical Microbiology·G CammarotaM Pistello
May 1, 1996·Journal of Virology·T TanakaK Shimotohno
May 19, 1997·Biochemical and Biophysical Research Communications·N J AdamsP Simmonds

❮ Previous
Next ❯

Citations

Oct 4, 2000·Reviews in Medical Virology·K L BarlowJ P Clewley
Jul 15, 2005·Expert Review of Molecular Diagnostics·Karen Weck
Apr 27, 2005·Journal of Viral Hepatitis·S BowdenC K Aitken
Feb 26, 2003·Journal of Medical Virology·Renata M PerezAntonio Eduardo B Silva
Dec 17, 2014·Journal of Clinical Microbiology·Suzanne EdwardsUNKNOWN Swiss HIV Cohort Study
Aug 14, 2002·World Journal of Gastroenterology : WJG·Song Chen, Yu-Ming Wang
Feb 3, 2000·Journal of Clinical Microbiology·P A WhiteW D Rawlinson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.