Hepatitis C virus infection and mixed cryoglobulinaemia: assessment of HCV RNA copy numbers in supernatant, cryoprecipitate and non-liver cells

Journal of Viral Hepatitis
A ManzinMassimo Clementi

Abstract

The aim of this study was to characterize hepatitis C virus (HCV) infection in patients with mixed cryoglobulinaemia (MC). The HCV RNA copy number was assayed in clinical specimens from 15 consecutive patients with MC and HCV infection. Absolute quantification of HCV RNA molecules was performed using a competitive reverse transcription-polymerase chain reaction (cRT-PCR). Specific HCV RNA sequences were detected and quantified in plasma samples from all patients (mean HCV RNA copy number 4.9 x 10(6) ml-1 plasma). A high concentration of HCV RNA molecules was detected in the cryoprecipitates of eight of the 15 patients, who had a cryoprecipitate/supernatant ratio higher than 3.0 (range 3.60 to 186.80): in the remaining seven patients this ratio was close to or lower than 1.0 (range 0.13 to 1.60). Quantitative analysis of HCV RNA molecules in cells other than hepatocytes (i.e. peripheral blood mononuclear cells (PBMCs) and bone marrow cells (BMCs), in which the HCV replicative intermediate was detected using strand specific RT-PCR, demonstrated that infection is detectable in nearly 60% of these extrahepatic cells. Quantitative analysis of HCV RNA in PBMCs and BMCs revealed low levels of viral nucleic acids.

References

Jun 30, 1977·The New England Journal of Medicine·Y LevoE C Franklin
Oct 1, 1992·Annals of Internal Medicine·R MisianiG Scudeller
Jul 1, 1992·Journal of Hepatology·A L ZignegoC Bréchot
Nov 19, 1992·The New England Journal of Medicine·V AgnelloL M Kaplan
Oct 17, 1992·Medicina clínica·J A Arzuaga TorreJ Martínez L de Letona
Jan 1, 1991·Magnetic Resonance Imaging·V RajanayagamJ C Gore
Apr 27, 1991·Lancet·M CasatoL Bonomo
Aug 1, 1990·The Journal of Infectious Diseases·M PascualJ A Schifferli
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·I SaitoY Ohta
Jan 1, 1968·International Archives of Allergy and Applied Immunology·O WagerE Klemola
Jan 1, 1995·Archives of Virology·M ClementiP Bagnarelli
Nov 1, 1993·Journal of Clinical Microbiology·E SiliniM U Mondelli
Feb 1, 1994·The American Journal of Medicine·P CacoubP Godeau
Aug 1, 1994·Journal of Clinical Microbiology·A ManzinM Clementi
Aug 1, 1995·Journal of Internal Medicine·C MazzaroG Pozzato
Aug 1, 1995·Scandinavian Journal of Immunology·V Agnello
Feb 18, 1993·The New England Journal of Medicine·R J JohnsonC E Alpers
Feb 1, 1993·PCR Methods and Applications·M ClementiP E Varaldo
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·J N SimonsM L Chalmers
Aug 1, 1994·Journal of Clinical Microbiology·N C Besnard, P M Andre
Sep 1, 1994·Gastroenterology·B F King
Nov 1, 1993·Gastroenterology·D A LevisonJ H Yardley
Apr 1, 1993·The Journal of General Virology·H M MüllerL Theilmann
Jan 1, 1962·The American Journal of Medicine·J LOSPALLUTOM ZIFF

❮ Previous
Next ❯

Citations

Mar 16, 2005·Biochemical and Biophysical Research Communications·Viviana FalcónVictor Tsutsumi
Dec 2, 1998·Journal of Hepatology·F Lunel, L Musset
Dec 4, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Thomas Watkins-RiedelMarkus Woegerbauer
Aug 21, 2009·Journal of Clinical Microbiology·Tony BrunsAndreas Stallmach
Jan 27, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·I RasulE J Heathcote
Aug 30, 2008·PloS One·José Manuel CuevasFernando González-Candelas
Apr 9, 1999·Sexually Transmitted Infections·G Rooney, R J Gilson
Feb 13, 2003·International Journal of Immunopathology and Pharmacology·R MannaG Gasbarrini
Mar 24, 2000·Journal of Clinical Gastroenterology·S A Sarbah, Z M Younossi
Jun 2, 2000·Journal of Clinical Microbiology·M Clementi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.