Hepatitis C Virus Lipoviroparticles Assemble in the Endoplasmic Reticulum (ER) and Bud off from the ER to the Golgi Compartment in COPII Vesicles

Journal of Virology
Gulam H SyedAleem Siddiqui

Abstract

Hepatitis C virus (HCV) exists as a lipoprotein-virus hybrid lipoviroparticle (LVP).In vitrostudies have demonstrated the importance of apolipoproteins in HCV secretion and infectivity, leading to the notion that HCV coopts the secretion of very-low-density lipoprotein (VLDL) for its egress. However, the mechanisms involved in virus particle assembly and egress are still elusive. The biogenesis of VLDL particles occurs in the endoplasmic reticulum (ER), followed by subsequent lipidation in the ER and Golgi compartment. The secretion of mature VLDL particles occurs through the Golgi secretory pathway. HCV virions are believed to latch onto or fuse with the nascent VLDL particle in either the ER or the Golgi compartment, resulting in the generation of LVPs. In our attempt to unravel the collaboration between HCV and VLDL secretion, we studied HCV particles budding from the ERen routeto the Golgi compartment in COPII vesicles. Biophysical characterization of COPII vesicles fractionated on an iodixanol gradient revealed that HCV RNA is enriched in the highly buoyant COPII vesicle fractions and cofractionates with apolipoprotein B (ApoB), ApoE, and the HCV core and envelope proteins. Electron microscopy of immunogold-labeled microse...Continue Reading

References

Jul 1, 1980·The Histochemical Journal·K T Tokuyasu
Jul 1, 1996·Histochemistry and Cell Biology·W LiouJ W Slot
Feb 25, 1999·Gastroenterology·T TakeuchiM Kohara
Mar 27, 2001·Current Opinion in Lipidology·G S Shelness, J A Sellers
Jan 19, 2002·Journal of Virology·Thomas C Mettenleiter
Dec 28, 2002·Journal of Virology·Iñigo J SalanuevaCristina Risco
Dec 15, 2004·Journal of Virology·Tomas LópezSusana López
Sep 8, 2006·Journal of Virology·Jin ZhongFrancis V Chisari
Mar 23, 2007·Proceedings of the National Academy of Sciences of the United States of America·Hua HuangJin Ye
Dec 14, 2007·Journal of Virology·Pablo GastaminzaFrancis V Chisari
Sep 18, 2008·The Journal of General Virology·Søren U NielsenGeoffrey L Toms
Oct 16, 2009·The Journal of General Virology·Lynsey CorlessMark Harris
Nov 9, 2010·The Journal of Biological Chemistry·Andreas MerzRalf Bartenschlager
Nov 26, 2010·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·D Lavanchy
Dec 15, 2010·Trends in Microbiology·Ralf BartenschlagerPatrice André
Apr 16, 2011·Nature Reviews. Microbiology·David C Johnson, Joel D Baines
Aug 23, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Gulam H Syed, Aleem Siddiqui
Sep 1, 2011·The EMBO Journal·Vivek Malhotra, Patrik Erlmann
Jan 14, 2012·PLoS Pathogens·Kelly E CollerGlenn Randall
Apr 12, 2012·Proceedings of the National Academy of Sciences of the United States of America·Erick GiangMansun Law
Jun 30, 2012·The Journal of Biological Chemistry·Bryan BishéAleem Siddiqui
Jul 4, 2012·The Journal of Clinical Investigation·Alanna StrongDaniel J Rader
Jan 9, 2013·The Journal of Biological Chemistry·Samata TiwariShadab A Siddiqi
May 22, 2013·Proceedings of the National Academy of Sciences of the United States of America·Maria Teresa CataneseCharles M Rice
Aug 8, 2014·Current Opinion in Lipidology·Alanna StrongDaniel J Rader
Jan 30, 2015·Journal of Virology·Ignacio BenedictoPedro L Majano
Feb 18, 2016·Proceedings of the National Academy of Sciences of the United States of America·Marion LussignolMaria Teresa Catanese
May 26, 2016·Liver International : Official Journal of the International Association for the Study of the Liver·David A SheridanJason Grebely

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Citations

Feb 9, 2018·International Journal of Molecular Sciences·Geng ChenYaping Wang
Mar 17, 2020·International Journal of Toxicology·Kenneth L HastingsGary R Burleson
Mar 6, 2018·Frontiers in Microbiology·Saba Valadkhan, Puri Fortes
Apr 16, 2020·Viruses·François-Loïc CossetSolène Denolly
Oct 17, 2017·Frontiers in Microbiology·Marina Barriocanal, Puri Fortes
Apr 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Romina MancinelliPiera Valenti
Apr 25, 2020·International Journal of Molecular Sciences·Hanna BleyEva Herker
Mar 4, 2020·Cold Spring Harbor Perspectives in Medicine·Kunitada Shimotohno
Jan 28, 2021·International Journal of Molecular Sciences·Ja Yeon Kim Chu, Jing-Hsiung James Ou
Mar 6, 2021·Journal of Cell Science·Miguel Hernandez-GonzalezMichael Way
Mar 18, 2021·Cell Reports·Irene AnastasiaLuca Pellegrini
Aug 3, 2021·Frontiers in Oncology·Pin ZhaoShaojun Xing
Aug 10, 2021·World Journal of Gastroenterology : WJG·Hui-Chun LiShih-Yen Lo
Jun 3, 2021·Journal of Medical Virology·Hanbin JiFen Huang

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