PMID: 7535737Apr 1, 1995Paper

Hepatitis C virus RNA and liver histology in blood donors reactive to a single antigen by second-generation recombinant immunoblot assay

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
A ZanellaM Pappalettera

Abstract

The clinical significance of single band reactivity (indeterminate pattern) at anti-hepatitis C virus (HCV) second-generation recombinant immunoblot assay (RIBA-2) was investigated in symptomless subjects with normal liver function tests to obtain data for their counseling and clinical management. Serum and hepatic HCV RNA were determined by the nested polymerase chain reaction, and liver histology was evaluated in 40 symptomless blood donors with stable indeterminate RIBA-2 pattern, including 38 reactive to c22-3. All but one had normal alanine aminotransferase (ALT) levels. Two new immunoblot tests, RIBA-3 and INNO-LIA HCV Ab III (LIA-III), which incorporate additional HCV antigens, were also done to assess whether they could identify the viremic subjects. Ten cases (25%, confidence interval 12 to 38) were HCV RNA positive. Three of the HCV RNA-positive and none of the HCV RNA-negative subjects had chronic hepatitis. RIBA-2 strong intensity of reaction (score > 2+) was observed in all the HCV RNA-positive and in 12 HCV RNA-negative subjects. RIBA-3 and LIA-III gave positive results in 9 of 10 and 10 of 10 HCV RNA-positive, but also in 8 of 30 and 24 of 30 HCV RNA-negative subjects. A c-22-3 reactivity score of 4+ by RIBA-3 an...Continue Reading

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Citations

Jul 1, 1996·Baillière's Clinical Gastroenterology·M Colombo
Jan 20, 2009·Journal of Medical Virology·Takashi KumadaMasashi Fujimori
Nov 21, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·David Van der PoortenJacob George
Dec 24, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·V DriesH P Dienes

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