Hepatitis C Virus RNA Strands Detection in Peripheral Blood Mononuclear Cells Legitimizes Virus Eradication in Negative Serum PCR Naïve and Post-treatment Patients

Journal of Clinical and Translational Hepatology
Mohamed Darwish Ahmed Abd Alla, Mostafa Kamel El Awady

Abstract

Background and Aims: Hepatitis C virus (HCV) hepatotropism is associated with intra-peripheral blood mononuclear cell (PBMC) infection that causes post-treatment relapse in RNA seronegative patients. Our understanding of the association of non-viremic hepatic fibrosis with positive anti-HCV IgG antibodies and active hepatocellular damage might be increased by PBMCs screening for intracellular infection. Thus, the goals of this study included evaluation of PBMCs PCR for diagnosing HCV infection, addressing PBMCs plus serum real-time (SRT) PCR benefits over SRT-PCR alone, studying intra-PBMCs distribution of RNA sense and antisense strands, and identifying treatment feasibility in solitary intracellular infection. Methods: Enzyme-linked immunosorbent assay, SRT-PCR and PBMCs PCR were used to evaluate HCV infection in 401 subjects. The patients were classified into groups of negative controls (n = 30), positive controls (n = 63), non-viremia post-treatment (experienced; n = 166) and naïve (n = 49) cases, and non-viremia positive PBMCs PCR naïve (n = 35) and experienced (n = 58) patients. Results: The diagnosis of true positive and negative by PBMCs PCR and SRT-PCR had 100% and 96.7% compatibility respectively. PBMCs PCR detected i...Continue Reading

References

Apr 1, 1993·The Journal of General Virology·H M MüllerL Theilmann
Oct 31, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T LaskusJ Rakela
Jul 15, 1999·Clinica Chimica Acta; International Journal of Clinical Chemistry·M K El-AwadyE A Zaki
Nov 21, 2008·Journal of Virology·Jeffrey WilkinsonTomasz Laskus
Apr 27, 2010·World Journal of Gastroenterology : WJG·Farah Bokharaei SalimFarhad Zamani
Sep 8, 2010·Expert Review of Anti-infective Therapy·Zania Stamataki
May 2, 2016·Archives of Virology·Mohammad-Navid BastaniShahin Fakhim

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Methods Mentioned

BETA
PCR
enzyme-linked immunosorbent assay

Software Mentioned

SSPS

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