PMID: 9557666Apr 29, 1998Paper

Hepatitis C virus structural proteins assemble into viruslike particles in insect cells

Journal of Virology
Thomas F BaumertT Jake Liang

Abstract

Hepatitis C virus (HCV) is a leading cause of chronic hepatitis in the world. The study of HCV has been hampered by the low level of viral particles in infected individuals, the inability to propagate efficiently the virus in cultured cells, and the lack of a convenient animal model. Due to these obstacles, neither the structure of the virus nor the prerequisites for its assembly have been clearly defined. In this report, we describe a model for the production and purification of HCV-like particles in insect cells using a recombinant baculovirus containing the cDNA of the HCV structural proteins. In insect cells, expressed HCV structural proteins assembled into enveloped viruslike particles (40 to 60 nm in diameter) in large cytoplasmic cisternae, presumably derived from the endoplasmic reticulum. Biophysical characterization of viruslike particles by CsCl and sucrose gradient centrifugation revealed biophysical properties similar to those of putative virions isolated from infected humans. The results suggested that HCV core and envelope proteins without p7 were sufficient for viral particle formation. Analysis of particle-associated nucleic acids demonstrated that HCV RNAs were selectively incorporated into the particles over ...Continue Reading

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Citations

Dec 25, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ming QiaoT Jake Liang
Jun 24, 2003·Reviews in Medical Virology·Anne Op De Beeck, Jean Dubuisson
Aug 13, 2003·The Journal of Biological Chemistry·Birke BartoschFrançois-Loïc Cosset
Aug 17, 2004·World Journal of Gastroenterology : WJG·Ying-Song WuMing Li
Mar 23, 2005·Liver International : Official Journal of the International Association for the Study of the Liver·Reginald F ClaytonArvind H Patel
Nov 22, 2005·The Journal of General Virology·Anuradha BalasubramanianRamesh K Ganju
Apr 22, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Marlène Dreux, Francois-Loïc Cosset
Aug 3, 2007·Journal of Gastroenterology·Philip Wintermeyer, Jack R Wands
Nov 3, 2011·Expert Review of Vaccines·Luigi BuonaguroFranco M Buonaguro
Nov 4, 2011·The Journal of General Virology·Victoria C EdwardsJonathan K Ball
Aug 12, 2014·World Journal of Gastroenterology : WJG·Filippo AnsaldiGiancarlo Icardi
Nov 4, 2015·World Journal of Hepatology·Jingya LyuKoji Murao
Jun 9, 2016·Expert Review of Vaccines·Catherine FauvelleThomas F Baumert
Jun 22, 2000·The Journal of General Virology·R Bartenschlager, V Lohmann
Oct 17, 2003·World Journal of Gastroenterology : WJG·Wei ZhaoShu-De Jiang
May 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Kazumoto MurataT Jake Liang
May 27, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Xinhua FangThomas F Baumert
Feb 11, 2005·Proceedings of the National Academy of Sciences of the United States of America·Theo HellerT Jake Liang
Jul 20, 2005·Expert Review of Vaccines·Geert Leroux-Roels
Feb 16, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Patrick MaillardAgata Budkowska
Mar 14, 2003·Journal of Virology·Stefan PöhlmannJane A McKeating
Aug 25, 2006·Journal of Virology·Heidi BarthThomas F Baumert

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