PMID: 9557649Apr 29, 1998Paper

Hepatitis delta antigen mediates the nuclear import of hepatitis delta virus RNA

Journal of Virology
H C ChouM M Lai

Abstract

Hepatitis delta virus (HDV) RNA replicates in the nuclei of virus-infected cells. The mechanism of nuclear import of HDV RNA is so far unknown. Using a fluorescein-labeled HDV RNA introduced into partially permeabilized HeLa cells, we found that HDV RNA accumulated only in the cytoplasm. However, in the presence of hepatitis delta antigen (HDAg), which is the only protein encoded by HDV RNA, the HDV RNA was translocated into the nucleus, suggesting that nuclear import of HDV RNA is mediated by HDAg. Deletion of the nuclear localization signal (NLS) or RNA-binding motifs of HDAg resulted in the failure of nuclear import of HDV RNA, indicating that both the NLS and an RNA-binding motif of HDAg are required for the RNA-transporting activity of HDAg. Surprisingly, any one of the three previously identified RNA-binding motifs was sufficient to confer the RNA-transporting activity. We have further shown that HDAg, via its NLS, interacts with karyopherin alpha2 in vitro, suggesting that nuclear import of the HDAg-HDV RNA complex is mediated by the karyopherin alpha2beta heterodimer. The nuclear import of HDV RNA may be the first biological function of HDAg in the HDV life cycle.

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Citations

Jan 1, 2010·Viruses·Valerie Greco-Stewart, Martin Pelchat
Dec 1, 2009·Viruses·Chung-Hsin Tseng, Michael M C Lai
Nov 8, 2013·Advances in Virology·Carolina AlvesCelso Cunha
Oct 1, 2010·Liver International : Official Journal of the International Association for the Study of the Liver·Stéphanie Pascarella, Francesco Negro
Aug 19, 2016·Nature Reviews. Gastroenterology & Hepatology·Florian A LemppStephan Urban
Mar 29, 2000·Critical Reviews in Clinical Laboratory Sciences·L E Modahl, M M Lai
Nov 4, 2016·The Journal of Biological Chemistry·Hsiu-Chen HuangCheng Huang
Nov 26, 2020·Viruses·Zhenfeng Zhang, Stephan Urban
Mar 29, 2018·Infectious Disorders Drug Targets·Yadira Lobaina, Yasser Perera
May 4, 2020·Antiviral Research·Julie Lucifora, Marion Delphin

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