Hepatitis delta virus RNA editing is highly specific for the amber/W site and is suppressed by hepatitis delta antigen.

Molecular and Cellular Biology
A G PolsonJohn L Casey

Abstract

RNA editing at adenosine 1012 (amber/W site) in the antigenomic RNA of hepatitis delta virus (HDV) allows two essential forms of the viral protein, hepatitis delta antigen (HDAg), to be synthesized from a single open reading frame. Editing at the amber/W site is thought to be catalyzed by one of the cellular enzymes known as adenosine deaminases that act on RNA (ADARs). In vitro, the enzymes ADAR1 and ADAR2 deaminate adenosines within many different sequences of base-paired RNA. Since promiscuous deamination could compromise the viability of HDV, we wondered if additional deamination events occurred within the highly base paired HDV RNA. By sequencing cDNAs derived from HDV RNA from transfected Huh-7 cells, we determined that the RNA was not extensively modified at other adenosines. Approximately 0.16 to 0.32 adenosines were modified per antigenome during 6 to 13 days posttransfection. Interestingly, all observed non-amber/W adenosine modifications, which occurred mostly at positions that are highly conserved among naturally occurring HDV isolates, were found in RNAs that were also modified at the amber/W site. Such coordinate modification likely limits potential deleterious effects of promiscuous editing. Neither viral replica...Continue Reading

References

Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J L CaseyJ L Gerin
Apr 1, 1992·Journal of Virology·W S RyuJ Taylor
Aug 1, 1992·Journal of Virology·H ZhengJ Taylor
May 18, 1989·Nature·S Kwok, R Higuchi
Mar 1, 1989·Journal of Virology·L SharmeenJ Taylor
Oct 1, 1986·The Journal of Infectious Diseases·K F Bergmann, J L Gerin
Sep 1, 1983·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M Rizzetto
Jan 1, 1995·Annual Review of Biochemistry·M M Lai
Mar 1, 1995·Journal of Virology·H J NetterJ M Taylor
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·M A O'Connell, W Keller
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J L CaseyJ L Gerin
May 1, 1993·Journal of Virology·D W Lazinski, J M Taylor
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·A HerbP H Seeburg
Mar 1, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·G A NiroJ L Casey
May 1, 1997·Trends in Biochemical Sciences·B L Bass

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Citations

Nov 28, 2015·Wiley Interdisciplinary Reviews. RNA·Sarah N Deffit, Heather A Hundley
Dec 3, 2015·PloS One·Eloy CuadradoTaco W Kuijpers
Jun 5, 2002·Annual Review of Biochemistry·Brenda L Bass
Nov 8, 2013·Advances in Virology·Carolina AlvesCelso Cunha
Mar 6, 2010·Advances in Bioinformatics·Chi-Ruei Huang, Szecheng J Lo
Jun 26, 2012·Clinics in Laboratory Medicine·Priya Abraham
Oct 26, 2002·Proceedings of the National Academy of Sciences of the United States of America·Swee Kee Wong, David W Lazinski
Jun 27, 2006·Journal of Pharmacological Sciences·Michihisa TohdaYasuyuki Nomura
Jan 1, 2010·Viruses·Renxiang ChenJohn L Casey
Jan 1, 2010·Viruses·Valerie Greco-Stewart, Martin Pelchat
Mar 1, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Sheng-Chieh HsuJaw-Ching Wu
Mar 27, 2007·Cold Spring Harbor Symposia on Quantitative Biology·B L Bass
Mar 29, 2000·Critical Reviews in Clinical Laboratory Sciences·L E Modahl, M M Lai
Mar 16, 2005·Molecular and Cellular Biology·Julie A LawBarbara Sollner-Webb
Oct 31, 2014·World Journal of Gastroenterology : WJG·Chi-Ruei Huang, Szecheng John Lo
Nov 4, 2016·The Journal of Biological Chemistry·Hsiu-Chen HuangCheng Huang
Nov 7, 2019·Wiley Interdisciplinary Reviews. RNA·Rachel Netzband, Cara T Pager
Sep 20, 2018·Viruses·Shachinthaka D Dissanayaka MudiyanselageYing Wang
Mar 23, 2007·Journal of Virology·Cheng HuangMing-Fu Chang
Feb 28, 2021·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Nikolaos I VlachogiannisDimitrios Paraskevis
Sep 15, 1998·Methods : a Companion to Methods in Enzymology·Y LiuC E Samuel
Nov 30, 2002·Biochimie·Myriam Schaub, Walter Keller

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