Hepatitis E virus enters liver cells through receptor-dependent clathrin-mediated endocytosis

Journal of Viral Hepatitis
N KapurS K Panda

Abstract

We investigated the virus-host interaction for hepatitis E virus (HEV) by performing competitive binding assays using in vitro assembled virus-like particles (VLPs). We used Escherichia coli expressed native capsid protein (pORF2) and its mutants with an attached Gly((5))-Ala (linker) reporter [enhanced green fluorescent protein (EGFP)/firefly luciferase (Fluc)]. Transmission electron microscopy and nanoparticle tracking showed near uniform particles of approximately 30-35 nm in diameter for pORF2 VLPs and 60-100 nm for reporter-linked VLPs. Binding of reporter-linked full-length (1-660aa) and N-terminal truncated (Δ1-112aa) pORF2 VLPs to Huh7 cell surfaces was found to be specific with 1.92 ± 0.065 × 10(5) sites per cell. Saturation binding indicated an equilibrium dissociation constant (K(d)) of 121.1 ± 23.83 and 123.8 ± 16.15 nm for pORF2-linker-EGFP and pORF2-linker-Fluc VLPs respectively. A similar binding pattern was observed for Δ1-112aa pORF2-linker-EGFP and Δ1-112aa pORF2-linker-Fluc VLPs with K(d) values of 123.6 ± 10.60 and 135.6 ± 16.19 nm respectively. The affinity (log K(i)) of pORF2 binding on Huh7 cells in the presence of EGFP-tagged and Fluc-tagged pORF2 VLPs was found to be approximately 2.0. However, no VLP f...Continue Reading

References

Mar 1, 1992·The Journal of Cell Biology·M T ShiehP G Spear
Feb 1, 1980·Journal of Virology·P L MarionW S Robinson
Mar 1, 1994·Clinical and Diagnostic Laboratory Immunology·M CarlG R Reyes
Jan 1, 1993·Archives of Virology. Supplementum·G R ReyesM A Purdy
Jan 1, 1997·Archives of Virology·T Krusat, H J Streckert
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·C M CarrP S Kim
Apr 4, 1998·Nucleic Acids Research·A Touze, P Coursaget
Mar 14, 1998·Protein Expression and Purification·R A RobinsonR H Purcell
Aug 8, 1998·Journal of Virology·A P Byrnes, D E Griffin
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·V N MalashkevichP S Kim
Aug 4, 2001·Biochemical and Biophysical Research Communications·S TyagiS K Lal
Jun 6, 2002·Microbes and Infection·Harald C WormGerald Brandstätter
Dec 19, 2002·Journal of Biomedicine & Biotechnology·Li XiaofangShahid Jameel
Jun 18, 2003·The Journal of General Virology·Shin-nosuke KanesashiHiroshi Handa
Jul 5, 2003·Journal of Tongji Medical University = Tong Ji Yi Ke Da Xue Xue Bao·S PengI H Frazer
Jul 18, 2003·The Journal of Biological Chemistry·Heidi BarthThomas F Baumert
Jan 7, 2004·Biochemical and Biophysical Research Communications·L GilbertC Oker-Blom
Aug 6, 2004·Protein Expression and Purification·M Reza Razeghifard
Nov 24, 2004·The Journal of Biological Chemistry·Shao-Wei LiNing-Shao Xia
Oct 20, 2006·Reviews in Medical Virology·Subrat Kumar PandaShagufta Rehman
Dec 13, 2006·Journal of Nanobiotechnology·Leona GilbertChristian Oker-Blom
Feb 28, 2007·The Journal of General Virology·Toshinori TanakaHiroaki Okamoto

❮ Previous
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Citations

Jan 1, 2014·Antiviral Research·Yannick Debing, Johan Neyts
Oct 30, 2012·Journal of Gastroenterology·Hiroaki Okamoto
Jun 16, 2015·Expert Opinion on Therapeutic Targets·Scott P Kenney, Xiang-Jin Meng
Feb 20, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Marco E FavrettoRoland Brock
Jun 25, 2015·Journal of Nanobiotechnology·Subrat Kumar PandaHemlata Durgapal
Dec 18, 2014·Future Microbiology·Harry R DaltonJacques Izopet
Nov 7, 2015·PloS One·Nikolai NikitinOlga Karpova
May 10, 2016·Expert Review of Gastroenterology & Hepatology·Rakesh Aggarwal, Amit Goel
Aug 23, 2016·Viruses·Xin YinZongdi Feng
Sep 23, 2016·Frontiers in Microbiology·Yuchen Nan, Yan-Jin Zhang
Sep 18, 2015·Microbiology and Molecular Biology Reviews : MMBR·Jan P Buchmann, Edward C Holmes
Mar 14, 2018·Cold Spring Harbor Perspectives in Medicine·Scott P Kenney, Xiang-Jin Meng
Jan 10, 2019·Médecine sciences : M/S·Maliki AnkavayLaurence Cocquerel
Nov 23, 2017·Nature Reviews. Gastroenterology & Hepatology·Ila NimgaonkarAlexander Ploss
Sep 25, 2019·Viruses·Xin Yin, Zongdi Feng
Mar 9, 2019·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Satish Chandrasekhar NairHalah Ibrahim
Jun 4, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Katie R YoungBrian J Ward
May 28, 2020·Cells·Noémie OechslinJérôme Gouttenoire
Jul 19, 2012·Macromolecular Bioscience·Sílvia A FerreiraFrancisco M Gama
Nov 19, 2019·Frontiers in Microbiology·Gayatri D KanadeYogesh A Karpe
Feb 9, 2019·Scientific Reports·Eun Byul LeeSeung Kew Yoon
Aug 24, 2020·Trends in Microbiology·Michael H WißingDaniel Todt
Jun 3, 2021·Journal of Medical Virology·Hanbin JiFen Huang

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