PMID: 9556759Apr 29, 1998Paper

Hepatocyte culture utilizing porous polyvinyl formal resin maintains long-term stable albumin secretion activity

Journal of Biomaterials Science. Polymer Edition
Hirotoshi MiyoshiNorio Ohshima

Abstract

To investigate the effects of culture conditions on the maintenance of metabolic functions of cultured hepatocytes, long-term hepatocyte culture lasting 20 days was performed under two different culture conditions, i.e. stationary cultures utilizing porous polymer (polyvinyl formal (PVF) resin) as a substratum and conventional monolayer dish cultures without PVF. Metabolic activities specific to hepatocytes were evaluated in terms of ammonia metabolism, urea synthesis, and albumin secretion. Concerning ammonia metabolic and urea synthetic activities, no significant differences in maintenance of these activities were found between the two culture conditions, and these activities rapidly decreased with the elapse of the culture period, especially during the early stage of the experiments. However, after day 10, these activities in the stationary cultures were maintained at a slightly more favorable level than in the monolayer cultures. On the other hand, compared with ammonia metabolism and urea synthesis, stable and well-maintained albumin secretion of hepatocytes (60% of the activity in day 1) was exhibited in the stationary culture experiments, despite that this particular activity under the monolayer culture condition gradual...Continue Reading

References

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Citations

Aug 5, 2000·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·H MiyoshiN Ohshima
Jun 13, 2002·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·John F PatzerRichard Miller
Aug 15, 2003·Journal of Biomedical Materials Research. Part a·Yascara Grisel Luna SaavedraFrancine Denizeau
Dec 16, 2005·Journal of Biomedical Materials Research. Part a·Tomo EhashiHirotoshi Miyoshi
Mar 16, 2013·Toxicology Research·Valerie Y SoldatowIvan Rusyn
Sep 15, 2001·Journal of Biomaterials Science. Polymer Edition·N KrastevaG Altankov
Jan 19, 2002·Annals of the New York Academy of Sciences·J F Patzer

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