Hepatocyte growth factor and inducible nitric oxide synthase are involved in multidrug resistance-induced angiogenesis in hepatocellular carcinoma cell lines

Cancer Research
Nadia LasagnaRoberto Mazzanti

Abstract

Based on literature, it is possible to hypothesize that multidrug resistance (MDR) and angiogenic phenotypes are linked to each other in human liver cancer cells. Our goal is to assess whether MDR cells trigger angiogenesis and to study the possible molecular mechanisms involved. Conditioned medium from parental drug-sensitive P5 cells (P5-CM) and MDR-positive P1(0.5) cells [P1(0.5)-CM] stimulated human umbilical vein endothelial cells (HUVEC) survival, proliferation, migration, and microtubular structure formation, but P1(0.5)-CM had a significantly greater effect than P5-CM. Cell implants were done in the rabbit avascular cornea to measure angiogenesis in vivo: P1(0.5) cells induced an important neovascular response in rabbit cornea after 1 week, whereas P5 cells had no effect. P1(0.5) and P5 cells produced vascular endothelial growth factor, but only P1(0.5) secreted hepatocyte growth factor (HGF) into the medium, and small interfering RNA specific for MDR1 clearly reduced HGF production in P1(0.5) cells. The transcription factor Ets-1 and the HGF receptor c-Met were up-regulated in P1(0.5) cells and in HUVEC cultured in P1(0.5)-CM. Inducible nitric oxide synthase (iNOS) seemed to play a major role in the proangiogenic effec...Continue Reading

References

Nov 1, 1976·British Journal of Cancer·G M MacNabJ M Urbanowicz
Apr 15, 1991·Biochemical and Biophysical Research Communications·K MatsumotoT Nakamura
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·D C JenkinsS Moncada
Jul 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R E Friesel, T Maciag
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D S GrantE M Rosen
Jan 1, 1993·Annual Review of Biochemistry·M M Gottesman, I Pastan
Jun 1, 1993·Journal of Hepatology·X ChenivesseC Bréchot
Oct 4, 1996·The Journal of Biological Chemistry·S E DunsmoreH G Welgus
Dec 31, 1997·The Journal of Clinical Investigation·T Michel, O Feron
Apr 29, 1998·Journal of the National Cancer Institute·O GalloM Ziche
May 23, 1998·Annual Review of Pharmacology and Toxicology·J R VaneR M Botting
Jan 27, 2000·Cell·D Hanahan, R A Weinberg
Nov 9, 2000·American Journal of Clinical Pathology·Y ItoN Matsuura
Aug 3, 2001·The Journal of Biological Chemistry·J SampathJ D Schuetz
Feb 21, 2002·World Journal of Gastroenterology : WJG·Q XieK Zhou
Feb 28, 2002·Molecular Cell·Xue WangReza Zarnegar
Mar 27, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ornella FantappièRoberto Mazzanti
Jun 5, 2002·World Journal of Gastroenterology : WJG·Lun-Xiu Qin, Zhao-You Tang
Jun 5, 2003·Nature Reviews. Cancer·Gabriele Bergers, Laura E Benjamin
Jun 5, 2003·Nature Medicine·Napoleone FerraraJennifer LeCouter
Nov 1, 2003·The International Journal of Biochemistry & Cell Biology·Shunichi ShimizuYuji Kiuchi
Dec 20, 2003·Nature Reviews. Molecular Cell Biology·Carmen BirchmeierGeorge F Vande Woude
Apr 23, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Fabio CianchiEmanuela Masini
Feb 3, 2005·Cell Research·Chong Feng Gao, George F Vande Woude

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Citations

Sep 28, 2006·Prostate Cancer and Prostatic Diseases·C CaiL Shemshedini
Apr 9, 2008·The Cancer Journal·Melanie B Thomas
Feb 1, 2008·Annals of Surgical Oncology·Melanie B ThomasPhilip Johnson
May 12, 2010·World Journal of Gastroenterology : WJG·Yuan ZhouBin Yan
Feb 20, 2009·Journal of Gastroenterology·Melanie Thomas
Feb 1, 2011·Drug Discoveries & Therapeutics·J J GaoW Tang
Aug 16, 2008·Biochemical and Biophysical Research Communications·Salvatore PatanéFlavio Maina
Dec 7, 2007·Molecular Aspects of Medicine·Roberto MazzantiLuigi Bolondi
Sep 8, 2007·Biochemical and Biophysical Research Communications·Juejin Wang, Shengnan Li
Jun 18, 2009·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Wei ZhangHou-Quan Tao
Oct 10, 2006·Experimental Cell Research·Michela SolazzoRoberto Mazzanti
Dec 12, 2012·Journal of Proteome Research·Jae-Sung YiYoung-Gyu Ko
Dec 1, 2011·Therapeutic Advances in Medical Oncology·Johann S de Bono, Timothy A Yap
Mar 2, 2016·World Journal of Experimental Medicine·Roberto MazzantiRenato Tassi
Aug 20, 2019·British Journal of Pharmacology·Ronghua WangTimothy R Billiar
Nov 30, 2013·BMB Reports·Deokbum ParkDooil Jeoung
Jun 27, 2019·The Journal of International Medical Research·Qing ChangXiao-Hui Zhao
Nov 6, 2020·Biochemical Society Transactions·Amir M AlsharabasyAbhay Pandit
May 19, 2007·Cancer Research·Ornella FantappièRoberto Mazzanti
Oct 3, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Luca Toschi, Pasi A Jänne
Feb 3, 2021·Oncogene·Georgina E WoodStéphanie Kermorgant

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