Hepatocyte growth factor improves survival after partial hepatectomy in cirrhotic rats suppressing apoptosis of hepatocytes

Surgery
Masayuki NishinoJiro Fujimoto

Abstract

Liver failure after hepatic resection is still a critical issue in the treatment of hepatic tumors in patients with liver cirrhosis. In the current study, the effect of hepatocyte growth factor (HGF) gene transfer, which is a multipotent growth factor, was examined in rats with liver cirrhosis that underwent 2/3 partial hepatectomy (PH). Rats were treated with 1 mL of 1% dimethylnitrosoamine (DMN) 3 consecutive days per week for 4 weeks and then received a 2/3 PH. Three days before the PH, human HGF gene plasmid (20 microg) encapsulated in hemagglutinating virus of Japan (HVJ)-liposome was administered through a direct injection in the portal vein. Control cirrhotic rats received empty HVJ-liposome in the same manner. HGF gene transfer significantly improved survival after PH in the cirrhotic rats, and it stimulated BrdU uptake in hepatocytes. Although the HGF gene transfer did not change the liver regeneration rate after PH, it suppressed hepatocyte apoptosis and upregulated an antiapoptotic protein, Bcl-xl, but it did not affect the expression of Bax, which is a proapoptotic protein. HGF gene transfer to cirrhotic livers improves liver failure-associated death after PH upregulating expression of an antiapoptotic protein, Bcl-xl.

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Citations

Dec 3, 2014·The International Journal of Biochemistry & Cell Biology·Grant J LoganGeorge C Yeoh
Jul 24, 2014·Clinical & Experimental Metastasis·Moritz J StrowitzkiMichael D Menger
Feb 4, 2010·World Journal of Gastroenterology : WJG·Tao HanZhen-Gang Zhao
Jan 19, 2010·Experimental and Molecular Pathology·Joan OlivaSamuel W French
Apr 21, 2009·Biomaterials·Caroline N JonesAlexander Revzin
Jun 22, 2012·Journal of Cellular Physiology·Balakrishnan Chakrapani NarmadaHanry Yu
Nov 20, 2013·Integrative Biology : Quantitative Biosciences From Nano to Macro·Dipali PatelAlexander Revzin
Oct 27, 2016·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Joohyun KimJohnny C Hong
Dec 20, 2011·World Journal of Gastrointestinal Pathophysiology·Stelios F AssimakopoulosChrisoula D Scopa

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