Hepatocyte growth factor induces transglutaminase activity that negatively regulates the growth signal in primary cultured hepatocytes

Experimental Cell Research
S KatohK Kitani

Abstract

Transglutaminase (TGase) activity increased 2.5-fold at 6 h after treatment of rat hepatocytes with 117 nM hepatocyte growth factor (HGF). In the same manner, putrescine incorporation into proteins of cells occurred in HGF-treated cells but did not in those pretreated with monodansylcadaverine (MDC), a TGase inhibitor, even in the presence of HGF. These results suggest that HGF-induced TGase was active and catalyzed some cross-linkage reaction. Cycloheximide completely blocked the increase in TGase activity induced by HGF, suggesting that HGF stimulated de novo synthesis of TGase within 6 h. Both [35S]methionine incorporation and Northern blotting analyses supported this possibility. Pretreatment of cells with MDC additionally increased HGF-induced DNA synthesis and the ratio of cells in S-phase. Similarly, TGase antisense oligonucleotide inhibited de novo synthesis of TGase, resulting in increase in the ratio of S-phase cells in the presence of HGF. Analyses of cross-linking of HGF to the receptor indicated that the antisense oligonucleotide inhibited the downregulation of HGF receptor subsequent to HGF-addition. These results provide the first evidence for inducibility of de novo synthesis of TGase by HGF and suggest that TGa...Continue Reading

Citations

Apr 1, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anthony J FilianoGail V W Johnson
Jul 12, 2003·American Journal of Respiratory Cell and Molecular Biology·Suman K BanerjeeMichael R Blackburn
May 19, 2006·Biomedical Research·Yosuke OhtakeYasuhito Ohkubo
Dec 9, 2004·Journal of Neurochemistry·Craig D C Bailey, Gail V W Johnson
Jun 19, 2004·The International Journal of Biochemistry & Cell Biology·Akira IchikawaKoji Ikura
Jun 18, 2002·Journal of Neurochemistry·Janusz Tucholski, Gail V W Johnson
Oct 19, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S K LeeS I Chung
Nov 17, 2007·Cell Biochemistry and Function·Yosuke OhtakeYasuhito Ohkubo
Dec 6, 2011·Amino Acids·Umberto TarantinoAugusto Orlandi
May 8, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yosuke OhtakeYasuhito Ohkubo
Dec 31, 1997·Biochimica Et Biophysica Acta·B M Fraij, R A Gonzales
Mar 23, 2004·Molecular and Cellular Neurosciences·Craig D C BaileyGail V W Johnson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.

Cell Signaling by Tyrosine Kinases

Receptor tyrosine kinases (RTKs) are the high-affinity cell surface receptors for many polypeptide growth factors, cytokines, and hormones. RTKs have been shown not only to be key regulators of normal cellular processes but also to have a critical role in the development and progression of many types of cancer. Discover the latest research on cell signaling and RTK here.