Hepatocyte growth factor protects human endothelial cells against advanced glycation end products-induced apoptosis

Biochemical and Biophysical Research Communications
Yi Jun ZhouJin Zhang

Abstract

Advanced glycation end products (AGEs) form by a non-enzymatic reaction between reducing sugars and biological proteins, which play an important role in the pathogenesis of atherosclerosis. In this study, we assessed AGEs effects on human umbilical vein endothelial cells (HUVECs) growth, proliferation and apoptosis. Additionally, we investigated whether hepatocyte growth factor (HGF), an anti-apoptotic factor for endothelial cells, prevents AGEs-induced apoptosis of HUVECs. HUVECs were treated with AGEs in the presence or absence of HGF. Treatment of HUVECs with AGEs changed cell morphology, decreased cell viability, and induced DNA fragmentation, leading to apoptosis. Apoptosis was induced by AGEs in a dose- and time-dependent fashion. AGEs markedly elevated Bax and decreased NF-kappaB, but not Bcl-2 expression. Additionally, AGEs significantly inhibited cell growth through a pro-apoptotic action involving caspase-3 and -9 activations in HUVECs. Most importantly, pretreatment with HGF protected against AGEs-induced cytotoxicity in the endothelial cells. HGF significantly promoted the expression of Bcl-2 and NF-kappaB, while decreasing the activities of caspase-3 and -9 without affecting Bax level. Our data suggest that AGEs in...Continue Reading

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Citations

Dec 28, 2006·Apoptosis : an International Journal on Programmed Cell Death·J H WangB Y Chen
Mar 19, 2015·Biomolecules·Kerstin NowotnyTilman Grune
Oct 4, 2014·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Xiao-Dan WuShi-Yuan Xu
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